Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9O79_RS18225 Genome accession   NZ_CP097721
Coordinates   3797878..3798318 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain MS1665     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3792878..3803318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O79_RS18205 (M9O79_18210) aroP 3793876..3795249 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M9O79_RS18210 (M9O79_18215) ampE 3795292..3796146 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  M9O79_RS18215 (M9O79_18220) ampD 3796143..3796694 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9O79_RS18220 (M9O79_18225) nadC 3796782..3797675 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M9O79_RS18225 (M9O79_18230) pilA 3797878..3798318 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  M9O79_RS18230 (M9O79_18235) pilB 3798328..3799713 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  M9O79_RS18235 (M9O79_18240) hofC 3799703..3800905 (+) 1203 WP_000157236.1 protein transport protein HofC -
  M9O79_RS18240 (M9O79_18245) guaC 3800940..3801983 (-) 1044 WP_001217338.1 GMP reductase -
  M9O79_RS18245 (M9O79_18250) - 3802139..3802183 (-) 45 WP_120795372.1 protein YacM -
  M9O79_RS18250 (M9O79_18255) coaE 3802208..3802828 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=596289 M9O79_RS18225 WP_000360895.1 3797878..3798318(+) (pilA) [Escherichia coli strain MS1665]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=596289 M9O79_RS18225 WP_000360895.1 3797878..3798318(+) (pilA) [Escherichia coli strain MS1665]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363