Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9O74_RS20485 Genome accession   NZ_CP097719
Coordinates   4175560..4176000 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain MS1679     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4170560..4181000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O74_RS20465 (M9O74_20460) aroP 4171561..4172931 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  M9O74_RS20470 (M9O74_20465) ampE 4172974..4173828 (-) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  M9O74_RS20475 (M9O74_20470) ampD 4173825..4174376 (-) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9O74_RS20480 (M9O74_20475) nadC 4174464..4175357 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M9O74_RS20485 (M9O74_20480) pilA 4175560..4176000 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  M9O74_RS20490 (M9O74_20485) pilB 4176010..4177395 (+) 1386 WP_001025165.1 type II secretion system protein GspE Machinery gene
  M9O74_RS20495 (M9O74_20490) hofC 4177385..4178587 (+) 1203 WP_000157293.1 protein transport protein HofC -
  M9O74_RS20500 (M9O74_20495) guaC 4178622..4179665 (-) 1044 WP_001217338.1 GMP reductase -
  M9O74_RS20505 (M9O74_20500) - 4179821..4179865 (-) 45 WP_120795372.1 protein YacM -
  M9O74_RS20510 (M9O74_20505) coaE 4179890..4180510 (+) 621 WP_001269512.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=596209 M9O74_RS20485 WP_000360904.1 4175560..4176000(+) (pilA) [Escherichia coli strain MS1679]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=596209 M9O74_RS20485 WP_000360904.1 4175560..4176000(+) (pilA) [Escherichia coli strain MS1679]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363