Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9O74_RS01490 Genome accession   NZ_CP097719
Coordinates   319652..321148 (+) Length   498 a.a.
NCBI ID   WP_001040613.1    Uniprot ID   -
Organism   Escherichia coli strain MS1679     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314652..326148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O74_RS01460 (M9O74_01460) tusA 314732..314977 (+) 246 WP_000130619.1 sulfurtransferase TusA -
  M9O74_RS01465 (M9O74_01465) zntA 315246..317444 (-) 2199 WP_000106587.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  M9O74_RS01470 (M9O74_01470) yhhN 317518..318144 (-) 627 WP_000964718.1 lysoplasmalogenase -
  M9O74_RS01475 (M9O74_01475) yhhM 318285..318644 (+) 360 WP_000042885.1 DUF2500 domain-containing protein -
  M9O74_RS01480 (M9O74_01480) yhhL 318647..318916 (-) 270 WP_001295207.1 DUF1145 family protein -
  M9O74_RS01485 (M9O74_01485) rsmD 318906..319502 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  M9O74_RS01490 (M9O74_01490) pilA 319652..321148 (+) 1497 WP_001040613.1 signal recognition particle-docking protein FtsY Machinery gene
  M9O74_RS01495 (M9O74_01495) ftsE 321151..321819 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  M9O74_RS01500 (M9O74_01500) ftsX 321812..322870 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  M9O74_RS01505 (M9O74_01505) rpoH 323115..323969 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  M9O74_RS01510 (M9O74_01510) livJ 324241..325344 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  M9O74_RS01515 (M9O74_01515) panM 325532..325915 (-) 384 WP_000778791.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54620.36 Da        Isoelectric Point: 4.1639

>NTDB_id=596154 M9O74_RS01490 WP_001040613.1 319652..321148(+) (pilA) [Escherichia coli strain MS1679]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIAQAQEPAKASEHAVEEQPQAHTEAEAETFAADVVEVSEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=596154 M9O74_RS01490 WP_001040613.1 319652..321148(+) (pilA) [Escherichia coli strain MS1679]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGCTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCTCTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAAGCGGTGATAGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCAACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAACCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTTTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGAGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378