Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9O77_RS19805 Genome accession   NZ_CP097716
Coordinates   4142373..4142813 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain MS1718     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4137373..4147813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O77_RS19785 (M9O77_19790) aroP 4138371..4139744 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M9O77_RS19790 (M9O77_19795) ampE 4139787..4140641 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  M9O77_RS19795 (M9O77_19800) ampD 4140638..4141189 (-) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9O77_RS19800 (M9O77_19805) nadC 4141277..4142170 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M9O77_RS19805 (M9O77_19810) pilA 4142373..4142813 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  M9O77_RS19810 (M9O77_19815) pilB 4142823..4144208 (+) 1386 WP_022645178.1 type II secretion system protein GspE Machinery gene
  M9O77_RS19815 (M9O77_19820) hofC 4144198..4145400 (+) 1203 WP_267314130.1 protein transport protein HofC -
  M9O77_RS19820 (M9O77_19825) guaC 4145435..4146478 (-) 1044 WP_001217337.1 GMP reductase -
  M9O77_RS19825 (M9O77_19830) - 4146634..4146678 (-) 45 WP_120795372.1 protein YacM -
  M9O77_RS19830 (M9O77_19835) coaE 4146703..4147323 (+) 621 WP_001269517.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=596125 M9O77_RS19805 WP_000360914.1 4142373..4142813(+) (pilA) [Escherichia coli strain MS1718]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=596125 M9O77_RS19805 WP_000360914.1 4142373..4142813(+) (pilA) [Escherichia coli strain MS1718]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363