Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9O76_RS01265 Genome accession   NZ_CP097711
Coordinates   274796..276292 (+) Length   498 a.a.
NCBI ID   WP_001040640.1    Uniprot ID   -
Organism   Escherichia coli strain MS1737     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269796..281292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O76_RS01235 (M9O76_01235) tusA 270043..270288 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  M9O76_RS01240 (M9O76_01240) zntA 270390..272588 (-) 2199 WP_001468058.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  M9O76_RS01245 (M9O76_01245) yhhN 272662..273288 (-) 627 WP_000964718.1 lysoplasmalogenase -
  M9O76_RS01250 (M9O76_01250) yhhM 273429..273788 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  M9O76_RS01255 (M9O76_01255) yhhL 273791..274060 (-) 270 WP_001295207.1 DUF1145 family protein -
  M9O76_RS01260 (M9O76_01260) rsmD 274050..274646 (-) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  M9O76_RS01265 (M9O76_01265) pilA 274796..276292 (+) 1497 WP_001040640.1 signal recognition particle-docking protein FtsY Machinery gene
  M9O76_RS01270 (M9O76_01270) ftsE 276295..276963 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  M9O76_RS01275 (M9O76_01275) ftsX 276956..278014 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  M9O76_RS01280 (M9O76_01280) rpoH 278259..279113 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  M9O76_RS01285 (M9O76_01285) dapA 279217..280101 (-) 885 WP_000661262.1 4-hydroxy-tetrahydrodipicolinate synthase -
  M9O76_RS01290 (M9O76_01290) - 280101..281021 (-) 921 WP_001332164.1 phosphoglycerate dehydrogenase -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54600.34 Da        Isoelectric Point: 4.1714

>NTDB_id=595985 M9O76_RS01265 WP_001040640.1 274796..276292(+) (pilA) [Escherichia coli strain MS1737]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVDIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=595985 M9O76_RS01265 WP_001040640.1 274796..276292(+) (pilA) [Escherichia coli strain MS1737]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGATATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375