Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   K4L72_RS08835 Genome accession   NZ_CP081304
Coordinates   1831648..1832082 (+) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus velezensis strain CL-4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1826648..1837082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4L72_RS08830 (K4L72_08830) - 1830148..1831359 (-) 1212 WP_057080788.1 cytochrome P450 -
  K4L72_RS08835 (K4L72_08835) nucA/comI 1831648..1832082 (+) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  K4L72_RS08840 (K4L72_08840) - 1832142..1832897 (+) 756 WP_003154084.1 YoaK family protein -
  K4L72_RS08845 (K4L72_08845) - 1832930..1833292 (-) 363 WP_003154082.1 hypothetical protein -
  K4L72_RS08850 (K4L72_08850) - 1833485..1834813 (-) 1329 WP_024085327.1 S8 family peptidase -
  K4L72_RS20375 (K4L72_08855) - 1834993..1835228 (+) 236 Protein_1707 hypothetical protein -
  K4L72_RS08860 (K4L72_08860) - 1835484..1836191 (+) 708 WP_079004849.1 poly-gamma-glutamate hydrolase family protein -
  K4L72_RS08865 (K4L72_08865) - 1836252..1836704 (+) 453 WP_014305039.1 OsmC family protein -
  K4L72_RS08870 (K4L72_08870) - 1836718..1837071 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=595901 K4L72_RS08835 WP_003154085.1 1831648..1832082(+) (nucA/comI) [Bacillus velezensis strain CL-4]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=595901 K4L72_RS08835 WP_003154085.1 1831648..1832082(+) (nucA/comI) [Bacillus velezensis strain CL-4]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486