Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M8341_RS01975 Genome accession   NZ_CP097709
Coordinates   433306..434658 (+) Length   450 a.a.
NCBI ID   WP_050157820.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428306..439658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8341_RS01955 (M8341_01955) - 429143..429709 (-) 567 WP_003117889.1 hypothetical protein -
  M8341_RS01960 (M8341_01960) rsmD 429712..430308 (-) 597 WP_009875718.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  M8341_RS01965 (M8341_01965) - 430308..431795 (-) 1488 WP_003459246.1 pitrilysin family protein -
  M8341_RS01970 (M8341_01970) - 431788..433185 (-) 1398 WP_003084498.1 pitrilysin family protein -
  M8341_RS01975 (M8341_01975) pilA 433306..434658 (+) 1353 WP_050157820.1 signal recognition particle-docking protein FtsY Machinery gene
  M8341_RS01980 (M8341_01980) ftsE 434655..435326 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  M8341_RS01985 (M8341_01985) ftsX 435326..436333 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  M8341_RS01990 (M8341_01990) rpoH 436447..437301 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  M8341_RS01995 (M8341_01995) - 437366..437971 (+) 606 WP_003084511.1 hypothetical protein -
  M8341_RS02000 (M8341_02000) mtgA 437976..438707 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  M8341_RS02005 (M8341_02005) - 438744..439121 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  M8341_RS02010 (M8341_02010) thiS 439224..439424 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 47886.29 Da        Isoelectric Point: 4.5795

>NTDB_id=595764 M8341_RS01975 WP_050157820.1 433306..434658(+) (pilA) [Pseudomonas aeruginosa strain PA-1]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPARQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIG
EGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDV
AREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQ
AAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLD
GTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=595764 M8341_RS01975 WP_050157820.1 433306..434658(+) (pilA) [Pseudomonas aeruginosa strain PA-1]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCGGCTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTCCGG
CAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGGCTGTCCAAGACCAGCGCCAGCATTGGC
GAAGGCATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGAC
CGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACA
GCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTC
GCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAA
GCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCT
GGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAG
GCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGA
GCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCA
CCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTTGCCCTGACCAAACTGGAC
GGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGG
GATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(160-225)

SRP54-type protein, GTPase domain (SRP54)

(245-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.454

96

0.456