Detailed information NTG0595666

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K3176_RS25120
• Genome accession: NZ_CP081202
• Coordinates: 5194080..5195228 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain P9W
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5189080..5200228

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K3176_RS25100 (K3176_25100)	-	5189818..5191002 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
K3176_RS25105 (K3176_25105)	-	5190999..5192372 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
K3176_RS25110 (K3176_25110)	-	5192615..5193019 (-)	405	WP_003084560.1	NINE protein	-
K3176_RS25115 (K3176_25115)	czcD	5193173..5194072 (+)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
K3176_RS25120 (K3176_25120)	pilU	5194080..5195228 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
K3176_RS25125 (K3176_25125)	pilT	5195406..5196440 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
K3176_RS25130 (K3176_25130)	-	5196655..5197347 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
K3176_RS25135 (K3176_25135)	proC	5197359..5198180 (+)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
K3176_RS25140 (K3176_25140)	-	5198191..5198784 (+)	594	WP_003084544.1	YggT family protein	-
K3176_RS25145 (K3176_25145)	-	5198781..5199077 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=595666 K3176_RS25120 WP_003110016.1 5194080..5195228(-) (pilU) [Pseudomonas aeruginosa strain P9W]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=595666 K3176_RS25120 WP_003110016.1 5194080..5195228(-) (pilU) [Pseudomonas aeruginosa strain P9W]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382