Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   K3169_RS02845 Genome accession   NZ_CP081201
Coordinates   638022..640241 (-) Length   739 a.a.
NCBI ID   WP_027901989.1    Uniprot ID   A0A2G0WFK1
Organism   Pseudomonas phytophila strain ICMP 23753     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 633022..645241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3169_RS02825 (K3169_02800) hslU 633092..634429 (-) 1338 WP_027901993.1 HslU--HslV peptidase ATPase subunit -
  K3169_RS02830 (K3169_02805) hslV 634498..635028 (-) 531 WP_027901992.1 ATP-dependent protease subunit HslV -
  K3169_RS02835 (K3169_02810) - 635250..635930 (-) 681 WP_255878355.1 SPOR domain-containing protein -
  K3169_RS02840 (K3169_02815) argS 635932..637668 (-) 1737 WP_263269880.1 arginine--tRNA ligase -
  K3169_RS02845 (K3169_02820) priA 638022..640241 (-) 2220 WP_027901989.1 primosomal protein N' Machinery gene
  K3169_RS02850 (K3169_02825) rpmE 640408..640641 (+) 234 WP_020293533.1 50S ribosomal protein L31 -
  K3169_RS02855 (K3169_02830) - 640653..641468 (+) 816 WP_263272198.1 thermonuclease family protein -
  K3169_RS02860 (K3169_02835) - 641760..643028 (+) 1269 WP_027901987.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81838.19 Da        Isoelectric Point: 8.2567

>NTDB_id=595622 K3169_RS02845 WP_027901989.1 638022..640241(-) (priA) [Pseudomonas phytophila strain ICMP 23753]
MPDAILRLALPSPLRRLFDYRAPAGVLRSALQPGMRLRVPFGRREMIGILIEIADHSEVPADKLKPAIALLDEQAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGEPAESRQERFWQVAPNASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKESLNLLLAKELVQVEVRSHAPSARHEHWLAQPELPLNTEQRAACEAIRAGFGSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRDRLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAQQPKFMRLDVKS
RPLDSGISGPMQQAIGQTLEAGQQVLVFLNRRGFAPTLLCHDCGWLSGCPRCDARMTVHQRSGELRCHHCGHVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGRVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEGFLDEACSVAEQLLNDMNLGGIELLGPVPAPMERRAGRYRAQLLLQSSARAPLHRLLASWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=595622 K3169_RS02845 WP_027901989.1 638022..640241(-) (priA) [Pseudomonas phytophila strain ICMP 23753]
GTGCCCGACGCCATTCTGCGCCTTGCCCTGCCTTCGCCACTGCGGCGCCTGTTCGACTACCGAGCACCTGCCGGAGTGCT
GCGCAGTGCCTTGCAGCCTGGCATGCGCCTGCGAGTCCCCTTCGGCCGTCGGGAAATGATCGGCATTCTGATCGAGATCG
CCGATCACAGCGAAGTGCCCGCAGACAAACTCAAGCCCGCCATCGCCCTGCTCGATGAGCAAGCCCCCCTGCCTCCCGCG
CTGTTCAAGCTGTGCCTGTGGACCTCTCAGTATTATCAGCACAGCCTCGGCGACACCTTGAGCTGGGCGCTGCCAGTCCT
GCTGCGCCAGGGCGAACCGGCCGAATCGCGCCAGGAGCGCTTCTGGCAGGTTGCGCCGAATGCCAGCGTCGATGACCCGC
GCATTGCCCGGGCACCCAAGCAACGCGAAGCACTGACCACCCTCGCCCAGCATCCACACGGTGTTGCGCATCAGTTGCTG
AGCAAGCTGATGCTTAATAAAGAAAGCCTCAATTTGCTGCTGGCTAAAGAGTTGGTGCAGGTTGAAGTACGCAGCCATGC
TCCTTCGGCGCGTCATGAACACTGGCTGGCCCAGCCGGAACTGCCCCTCAACACGGAACAACGCGCGGCCTGCGAGGCGA
TTCGCGCCGGGTTCGGCAGCTTTCATGCGTTCCTGCTGGCGGGCGTTACCGGCAGCGGCAAGACCGAAGTCTATTTACAG
CTGATCCGCGAAACGCTGGAAGCCGGTAAACAGGCGCTGGTGTTGATCCCGGAAATCAACCTCGGGCCGCAAACCCTGGC
GCGCTTCGAGCAGCGCTTCAACGCACGCATCGCCCTGCTGCATTCGGCAGTCAATGATCGTGACCGGCTGGATGCCTGGC
TGGCAGCGCGGGATGGCGAGGCCGATATTATTATCGGTACTCGTTCGGCGCTGTTCACGCCGATGAAGAATCCCGGCCTG
ATCATCATCGACGAAGAGCACGACGGCTCCTATAAACAGCAGGAAGGCCTGCGCTATCACGCACGGGATCTGGCTTTAGT
GCGTGCACGCCAGGAAAATATCCCCATTGTGCTCGGCTCGGCCACACCTTCGCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTACGGCCTGTTGCGCCTCAATGAGCGTGCGGGCGGCGCGCAGCAACCGAAATTCATGCGGCTGGACGTTAAAAGC
CGTCCACTGGACAGCGGTATCTCCGGACCGATGCAACAAGCCATCGGCCAGACTCTCGAAGCCGGTCAGCAAGTGTTGGT
CTTCCTCAACCGACGCGGCTTTGCGCCGACGCTGCTGTGCCACGATTGCGGCTGGCTGTCGGGCTGCCCGCGATGTGATG
CGCGAATGACCGTGCATCAGCGCTCCGGGGAATTGCGCTGCCACCACTGCGGCCACGTCGAACGCGTACCGCGCCAGTGC
CCGTCCTGCGGCAAAGTGGATTTGCGCCCGGTTGGCGCAGGCACCGAGCGCGCTGAAGAGCGTCTGGCGATTCTGTTTCC
GGATTACCCCGTATTGCGGGTTGATCGCGACAGCACCTCGCGCAAAGACGCGATGAATCAACTGTTCGCGACCATTCAGC
GCGGCCAGCCGTGCATTCTGGTGGGCACACAGATGCTCGCCAAAGGGCACCACTTCCCGCGTGTGACACTGGTGTCGATT
CTGGATGCCGATGGCGGCCTGTTTTCCGGAGACTTCCGTGCCAGCGAGCGCATGGCGCAGCTGATCGTTCAGGTTGCCGG
GCGGGCCGGACGGGCTGAAGAGCCAGGCCGGGTGATCATCCAGACGCACCTGGCGGATCACCCACTGCTGATTCAGCTTA
CCGAACAAGGTTACTTCGCCTTCGCCGAGCAAGCCCTGAGCGAACGTCGCGCGGCTGGTTTGCCACCTTTTGCGCATCTG
GCACTGTTACGGGCCGAAGCCCATAAACCGGGTCAGGCCGAAGGGTTTCTCGATGAGGCCTGCTCGGTTGCCGAGCAGCT
GTTGAACGACATGAATCTGGGCGGCATTGAATTGCTCGGCCCGGTCCCGGCACCGATGGAGCGCCGCGCCGGTCGATATC
GAGCACAATTGCTGCTGCAATCCAGCGCCCGAGCGCCACTGCACCGGCTGCTTGCTTCCTGGCTGCTGGCGCTGGAGCAA
ATGCCGAGCGGCAGGCAGGTCAGGTGGTCGCTGGATGTGGATCCGGTGGATTTGTATTAG

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G0WFK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48