Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K4H28_RS02690 Genome accession   NZ_CP081150
Coordinates   568676..570058 (+) Length   460 a.a.
NCBI ID   WP_221006843.1    Uniprot ID   -
Organism   Deefgea tanakiae strain D25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 563676..575058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4H28_RS02665 (K4H28_02665) - 565271..566263 (+) 993 WP_221006834.1 KpsF/GutQ family sugar-phosphate isomerase -
  K4H28_RS02670 (K4H28_02670) kdsC 566346..566861 (+) 516 WP_221006836.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  K4H28_RS02675 (K4H28_02675) lptC 566871..567467 (+) 597 WP_221006838.1 LPS export ABC transporter periplasmic protein LptC -
  K4H28_RS02680 (K4H28_02680) lptA 567424..567918 (+) 495 WP_255573598.1 lipopolysaccharide transport periplasmic protein LptA -
  K4H28_RS02685 (K4H28_02685) lptB 567915..568637 (+) 723 WP_221006842.1 LPS export ABC transporter ATP-binding protein -
  K4H28_RS02690 (K4H28_02690) rpoN/rpoN1 568676..570058 (+) 1383 WP_221006843.1 RNA polymerase factor sigma-54 Machinery gene
  K4H28_RS02695 (K4H28_02695) hpf 570105..570431 (+) 327 WP_221006845.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  K4H28_RS02700 (K4H28_02700) ptsN 570536..571006 (+) 471 WP_221006847.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K4H28_RS02705 (K4H28_02705) hprK 571133..572077 (+) 945 WP_221006850.1 HPr(Ser) kinase/phosphatase -
  K4H28_RS02710 (K4H28_02710) rapZ 572077..572934 (+) 858 WP_221006851.1 RNase adapter RapZ -
  K4H28_RS02715 (K4H28_02715) - 572960..573322 (+) 363 WP_221006852.1 NusG domain II-containing protein -
  K4H28_RS02720 (K4H28_02720) - 573303..573851 (+) 549 WP_221006854.1 Gx transporter family protein -
  K4H28_RS02725 (K4H28_02725) - 573892..574491 (+) 600 WP_221006856.1 flavin prenyltransferase UbiX -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52179.39 Da        Isoelectric Point: 4.5566

>NTDB_id=595379 K4H28_RS02690 WP_221006843.1 568676..570058(+) (rpoN/rpoN1) [Deefgea tanakiae strain D25]
MKQTLQLRMSQQLNLTPQLQQSIKLLQLSTLEFQQEIEQFLAENPMLEREDGVVDAGEEAAPLEVSEVVEEGDSLRWDEV
RSGQSYDEDDENDPALRVQKTYSLRDYLLEQSGCLVVSERDKALINLLIDALDEDGLLSQTLDEIYAQIPEELITELELE
PEELQIALQHLQQLEPLGVGARDLAESLTIQLRVLPDSPVRQIAIRLVNEGLDLLAQRDYTRLKRQLQCDETTLRDAQAL
VRGLTPRPGANWSSETTRYVVPDVIVTKVRGQWQVRLNFAAQPKLKVNEMYAKLLQGDSTMGLSGKLQEARWLIKSVEQR
GSTILRVAEAIVVRQKAFFEYGEVAMRPLVLRDIADELELHESTISRVTTQKYMLTPRGVFEYKYFFGSHVESETGGENS
ATSIKALIKQLVQQENTHKPLSDSAITEELARQGVVVARRTVAKYREALMIPPVNQRKCL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=595379 K4H28_RS02690 WP_221006843.1 568676..570058(+) (rpoN/rpoN1) [Deefgea tanakiae strain D25]
ATGAAGCAAACCCTGCAGCTGCGCATGTCGCAGCAGCTTAATCTAACACCGCAACTTCAGCAGTCAATCAAACTGTTGCA
ACTCTCTACGCTTGAATTTCAACAAGAAATTGAACAGTTCCTCGCTGAAAATCCGATGTTAGAGCGTGAGGATGGCGTGG
TTGATGCGGGTGAAGAAGCTGCGCCGCTCGAAGTCAGCGAAGTCGTTGAAGAGGGGGATAGCCTGCGCTGGGACGAGGTC
AGATCAGGTCAATCGTATGACGAAGATGATGAAAATGACCCTGCTTTAAGGGTTCAAAAAACGTATTCTTTGCGCGACTA
TTTGCTTGAACAAAGTGGCTGTTTGGTGGTCTCAGAGCGGGATAAGGCGTTGATTAATTTGTTAATTGATGCCTTGGATG
AGGACGGTTTGCTAAGCCAAACGTTAGATGAAATCTATGCTCAGATCCCCGAAGAGCTGATCACTGAGTTGGAGTTAGAG
CCAGAAGAGCTGCAAATTGCATTGCAGCATTTGCAGCAACTTGAGCCTTTGGGTGTTGGCGCAAGGGATTTAGCCGAGTC
TTTGACAATACAGCTCCGCGTTTTGCCCGATAGTCCAGTGAGACAAATTGCCATTCGATTAGTGAATGAAGGACTCGATT
TATTGGCGCAGCGGGATTACACTAGACTTAAGCGTCAACTGCAATGCGATGAAACAACGCTGCGAGATGCGCAAGCTTTG
GTGCGTGGATTAACGCCAAGACCGGGGGCAAATTGGTCGTCTGAGACGACTCGCTATGTAGTTCCAGATGTCATTGTGAC
AAAAGTGCGTGGGCAATGGCAGGTGCGCTTGAATTTTGCGGCGCAGCCCAAACTTAAAGTCAATGAGATGTACGCCAAGC
TGTTGCAAGGTGATTCCACAATGGGTTTGAGTGGAAAATTGCAAGAAGCTCGTTGGTTGATTAAAAGTGTTGAACAGCGT
GGCAGTACGATTTTACGTGTTGCTGAAGCGATTGTGGTACGGCAAAAAGCGTTTTTTGAGTATGGTGAAGTGGCAATGCG
GCCCTTGGTGTTGCGTGATATTGCGGATGAGTTAGAGTTGCATGAATCCACCATTTCACGGGTGACAACGCAAAAATACA
TGCTGACGCCACGAGGGGTATTTGAGTACAAATATTTCTTTGGTAGTCATGTTGAAAGCGAAACGGGTGGTGAAAATTCA
GCAACATCCATCAAAGCTTTGATCAAACAACTGGTACAGCAAGAAAATACGCACAAACCTTTATCGGATAGTGCTATAAC
TGAAGAACTCGCACGGCAAGGTGTGGTGGTTGCGCGACGCACCGTTGCTAAGTACAGGGAGGCATTAATGATTCCTCCCG
TTAATCAGCGCAAGTGTTTGTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(304-458)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(98-291)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.165

100

0.576