Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K4H25_RS13500 Genome accession   NZ_CP081149
Coordinates   2892268..2893650 (-) Length   460 a.a.
NCBI ID   WP_221020971.1    Uniprot ID   -
Organism   Deefgea piscis strain D13     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2887268..2898650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4H25_RS13465 (K4H25_13465) - 2887896..2888498 (-) 603 WP_221020968.1 flavin prenyltransferase UbiX -
  K4H25_RS13470 (K4H25_13470) - 2888495..2889034 (-) 540 WP_221020969.1 Gx transporter family protein -
  K4H25_RS13475 (K4H25_13475) - 2889015..2889422 (-) 408 WP_255587677.1 NusG domain II-containing protein -
  K4H25_RS13480 (K4H25_13480) rapZ 2889400..2890263 (-) 864 WP_221020970.1 RNase adapter RapZ -
  K4H25_RS13485 (K4H25_13485) hprK 2890263..2891207 (-) 945 WP_173532047.1 HPr(Ser) kinase/phosphatase -
  K4H25_RS13490 (K4H25_13490) ptsN 2891320..2891793 (-) 474 WP_173534556.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K4H25_RS13495 (K4H25_13495) hpf 2891898..2892224 (-) 327 WP_173532048.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  K4H25_RS13500 (K4H25_13500) rpoN/rpoN1 2892268..2893650 (-) 1383 WP_221020971.1 RNA polymerase factor sigma-54 Machinery gene
  K4H25_RS13505 (K4H25_13505) lptB 2893679..2894401 (-) 723 WP_221020972.1 LPS export ABC transporter ATP-binding protein -
  K4H25_RS13510 (K4H25_13510) lptA 2894398..2894892 (-) 495 WP_221020973.1 lipopolysaccharide transport periplasmic protein LptA -
  K4H25_RS13515 (K4H25_13515) lptC 2894849..2895445 (-) 597 WP_221020974.1 LPS export ABC transporter periplasmic protein LptC -
  K4H25_RS13520 (K4H25_13520) - 2895455..2895970 (-) 516 WP_173532053.1 HAD family hydrolase -
  K4H25_RS13525 (K4H25_13525) - 2896063..2897049 (-) 987 WP_221022955.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52062.38 Da        Isoelectric Point: 4.6777

>NTDB_id=595372 K4H25_RS13500 WP_221020971.1 2892268..2893650(-) (rpoN/rpoN1) [Deefgea piscis strain D13]
MKQTLQLRMSQQLNLTPQLQQSIKLLQLSTLDFQQEIEQFLAENPMLEREDGVQDAGEEAEKPEVSEIVEDGDSLRWDEV
RSNASFDEGDENDPALRVAKLYSLRDYLLAQTGCLPLSDRDSALISLLIDALDDDGMLSQSLEEIYAQLPESLVQELELE
PEELQIALQHLQQLEPLGVGARDLPESLTLQLRVLPENSIRNIAIRLVNEGLDLLAQRDYTRLKRQLQCDEVALKNAQIL
IRRLTPRPGANWSAETTRYVLPDVIVTKVRGQWQVRLNSAAQPKLRVNELYAKLLQGDSAAALSGKLQEARWLIKSVEQR
GSTILRVAEAIVMRQKAFFEYGEVAMRPLVLRDIADELELHESTISRVTTQKYMLTPRGVFEYKYFFGSHVESESGGENS
ATSIKALIKQLVQQENQHKPLSDSAITEELARQGVVVARRTVAKYREALMIPPVNLRKCL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=595372 K4H25_RS13500 WP_221020971.1 2892268..2893650(-) (rpoN/rpoN1) [Deefgea piscis strain D13]
ATGAAGCAAACCTTGCAGTTGCGGATGTCGCAACAGCTCAATTTGACACCGCAGCTTCAGCAATCGATTAAGCTTTTGCA
GTTGTCTACGCTTGATTTCCAACAAGAAATAGAGCAATTTTTAGCCGAAAATCCCATGCTTGAACGCGAAGATGGCGTGC
AAGATGCGGGTGAGGAAGCTGAAAAGCCTGAGGTCAGTGAGATCGTTGAAGATGGCGATAGCTTGCGCTGGGATGAGGTG
CGCAGTAACGCCTCATTTGATGAGGGTGATGAAAATGACCCCGCTTTGCGGGTGGCGAAGCTGTACTCGTTGCGTGATTA
TTTGTTAGCGCAAACTGGCTGCTTACCCTTATCTGATCGTGATAGCGCATTGATTTCATTGCTGATTGATGCGCTAGATG
ATGACGGAATGTTAAGTCAGAGCTTGGAAGAAATTTACGCGCAGCTACCAGAGAGCTTGGTGCAAGAACTTGAGCTGGAA
CCAGAAGAATTACAAATCGCACTGCAACATTTACAGCAACTTGAGCCACTCGGTGTCGGTGCACGTGATTTGCCCGAGTC
GTTAACATTGCAATTGCGGGTCTTGCCAGAAAATAGTATTCGAAATATTGCCATTCGATTGGTGAATGAAGGTCTCGATT
TATTGGCGCAACGGGATTACACTAGACTTAAACGTCAACTGCAGTGCGATGAAGTGGCACTCAAAAACGCGCAAATATTA
ATTCGGCGTTTAACACCAAGACCGGGTGCAAATTGGTCAGCAGAGACGACACGCTACGTTTTACCTGATGTGATTGTGAC
CAAGGTGCGCGGGCAATGGCAGGTACGCTTAAATAGCGCGGCGCAGCCCAAGCTAAGAGTCAATGAATTGTATGCAAAGT
TACTACAAGGCGACTCAGCAGCCGCGCTCAGTGGTAAGCTACAAGAAGCACGCTGGCTGATTAAAAGTGTTGAGCAACGA
GGCAGTACGATTTTACGGGTCGCTGAAGCCATTGTAATGCGACAAAAAGCATTTTTTGAGTATGGTGAAGTTGCAATGCG
GCCTTTAGTGCTGCGCGATATTGCGGATGAACTTGAATTACATGAGTCGACGATTTCTCGGGTAACAACGCAAAAATACA
TGCTGACCCCGCGCGGTGTTTTTGAGTACAAATACTTTTTTGGCAGTCATGTTGAAAGTGAAAGCGGTGGTGAAAACTCA
GCAACATCGATTAAGGCTTTGATCAAACAACTGGTACAGCAAGAAAATCAGCACAAGCCATTATCCGATAGTGCTATAAC
TGAAGAACTCGCACGGCAAGGTGTTGTGGTTGCGCGGCGCACCGTTGCTAAGTACAGGGAGGCATTAATGATTCCTCCCG
TTAATCTGCGCAAGTGCTTATAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(101-291)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.174

100

0.574