Detailed information NTG0595349

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K4H25_RS00945
• Genome accession: NZ_CP081149
• Coordinates: 204587..205771 (+)
• Length: 394 a.a.
• NCBI ID: WP_221021621.1
• Uniprot ID: -
• Organism: Deefgea piscis strain D13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 199587..210771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K4H25_RS00930 (K4H25_00930)	-	199996..200685 (-)	690	WP_221021618.1	metallophosphoesterase	-
K4H25_RS00935 (K4H25_00935)	-	200900..202153 (+)	1254	WP_221021619.1	Hsp70 family protein	-
K4H25_RS00940 (K4H25_00940)	-	202485..204347 (+)	1863	WP_221021620.1	DEAD/DEAH box helicase	-
K4H25_RS00945 (K4H25_00945)	pilU	204587..205771 (+)	1185	WP_221021621.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K4H25_RS00950 (K4H25_00950)	-	205768..206265 (-)	498	WP_221021622.1	GNAT family N-acetyltransferase	-
K4H25_RS00955 (K4H25_00955)	-	206442..206981 (-)	540	WP_221021623.1	DUF1523 family protein	-
K4H25_RS00960 (K4H25_00960)	-	207314..207643 (+)	330	WP_173532647.1	zinc ribbon domain-containing protein YjdM	-
K4H25_RS00965 (K4H25_00965)	gnd	207774..208685 (-)	912	WP_221021624.1	phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating)	-
K4H25_RS00970 (K4H25_00970)	-	208868..209881 (+)	1014	WP_173532649.1	peptidase U32 family protein	-

Sequence

Protein

• Length: 394 a.a.
• Molecular weight: 44075.72 Da
• Isoelectric Point: 6.3928

>NTDB_id=595349 K4H25_RS00945 WP_221021621.1 204587..205771(+) (pilU) [Deefgea piscis strain D13]
MSPEQAKKTMHDLLRMMIQHKASDLFIADDFPPAMKINGRMTPIGAQKLNAAATQALALAIMRADQQTEFAKEFECNFAI
NPEGIGRFRVNVFMQQEKVGMVLRTITSKIPNFDELGLPKVLKEVMMSKRGLVLLVGGTGSGKSTTMAAMIDYRNENSHG
HIITIEDPIEYVHKNKNCLVTHREVGRDTKNWFAALKNTLRQAPDVILIGEIRDRETMEYAMAFAETGHLCMGTLHANSS
NQALERIVNFFPEERHPQLYMDLSLNMRGIISQRLVATPDGKGRSAAIEILLNTPLVADMIFKGDVGGVKTAMAKSKELG
MQTFDMALFELYEAGKISMEEALKNADSLNELRLNIKLNSLHHRKEEHHSGLEHLTLEAQQEPEEEAEATESTN

Nucleotide

• Length: 1185 bp

>NTDB_id=595349 K4H25_RS00945 WP_221021621.1 204587..205771(+) (pilU) [Deefgea piscis strain D13]
ATGAGCCCCGAACAAGCTAAAAAAACCATGCATGACTTACTGCGGATGATGATTCAACACAAAGCATCCGATTTGTTTAT
TGCCGATGATTTTCCGCCAGCCATGAAGATCAACGGCCGAATGACGCCGATTGGGGCACAAAAACTCAATGCCGCAGCCA
CGCAAGCGCTGGCTTTGGCCATTATGCGAGCCGATCAACAAACCGAGTTTGCTAAAGAATTTGAATGCAATTTTGCCATT
AACCCCGAAGGCATCGGCCGCTTTCGGGTTAATGTCTTTATGCAGCAAGAAAAGGTCGGCATGGTGCTGCGAACCATCAC
CAGCAAGATTCCTAATTTTGATGAATTAGGACTGCCCAAAGTATTAAAAGAAGTGATGATGAGTAAGCGCGGTTTAGTGC
TATTGGTTGGCGGCACCGGCTCGGGTAAATCAACGACGATGGCGGCGATGATTGATTACCGCAATGAAAACAGCCACGGC
CACATCATTACCATCGAAGACCCAATCGAGTATGTGCATAAAAATAAAAACTGCTTGGTGACGCACCGTGAAGTGGGGCG
TGATACCAAAAACTGGTTTGCTGCGCTCAAAAACACGCTGCGCCAAGCGCCAGATGTGATTTTAATTGGCGAAATTCGCG
ACCGAGAAACCATGGAATACGCCATGGCGTTTGCTGAAACCGGCCATCTTTGCATGGGCACTTTGCACGCCAATAGTTCC
AATCAAGCTTTGGAGCGAATTGTTAATTTCTTCCCTGAAGAGCGCCACCCGCAGCTGTATATGGATTTATCGCTCAATAT
GCGCGGTATTATTTCGCAGCGCTTGGTAGCCACTCCCGATGGCAAAGGGCGTAGCGCCGCAATTGAAATCTTGCTCAATA
CGCCGCTGGTAGCCGATATGATTTTTAAAGGTGACGTGGGCGGCGTTAAAACTGCAATGGCCAAGTCCAAAGAACTGGGA
ATGCAAACCTTTGATATGGCGCTGTTTGAACTGTATGAGGCGGGCAAAATCAGTATGGAAGAGGCGCTCAAAAACGCTGA
TTCACTCAATGAGCTGCGCTTAAATATTAAGCTCAACAGCTTGCATCATCGTAAAGAAGAGCATCACAGCGGGCTTGAGC
ATCTCACGCTTGAAGCACAGCAAGAGCCCGAAGAAGAGGCAGAGGCTACCGAAAGCACCAACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (86-276)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.621	88.325	0.518
pilU	Acinetobacter baylyi ADP1	56.41	89.086	0.503
pilU	Vibrio cholerae strain A1552	53.521	90.102	0.482
pilT	Pseudomonas aeruginosa PAK	43.284	85.025	0.368
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.136	85.533	0.36