Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K3M67_RS10630 Genome accession   NZ_CP081001
Coordinates   2136323..2137690 (-) Length   455 a.a.
NCBI ID   WP_285831457.1    Uniprot ID   A0A9Y2F1B8
Organism   Sphingobium sp. V4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2131323..2142690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3M67_RS10600 (K3M67_10600) - 2131416..2132033 (+) 618 WP_066857831.1 TetR family transcriptional regulator -
  K3M67_RS10605 (K3M67_10605) obgE 2132064..2133164 (+) 1101 WP_066857828.1 GTPase ObgE -
  K3M67_RS10610 (K3M67_10610) proB 2133196..2134326 (+) 1131 WP_285831455.1 glutamate 5-kinase -
  K3M67_RS10615 (K3M67_10615) - 2134350..2135246 (+) 897 WP_285832928.1 NAD(P)H-binding protein -
  K3M67_RS10620 (K3M67_10620) - 2135265..2135786 (-) 522 WP_285831456.1 hypothetical protein -
  K3M67_RS10625 (K3M67_10625) - 2135790..2136311 (-) 522 WP_066857822.1 CvpA family protein -
  K3M67_RS10630 (K3M67_10630) radA 2136323..2137690 (-) 1368 WP_285831457.1 DNA repair protein RadA Machinery gene
  K3M67_RS10635 (K3M67_10635) - 2137779..2138477 (+) 699 WP_066857819.1 endonuclease/exonuclease/phosphatase family protein -
  K3M67_RS10640 (K3M67_10640) - 2138529..2140862 (+) 2334 WP_066857816.1 patatin-like protein -
  K3M67_RS10645 (K3M67_10645) - 2140892..2141359 (+) 468 WP_285832929.1 hypothetical protein -
  K3M67_RS10650 (K3M67_10650) - 2141386..2141676 (-) 291 WP_285831458.1 4a-hydroxytetrahydrobiopterin dehydratase -
  K3M67_RS10655 (K3M67_10655) - 2141776..2142171 (+) 396 WP_285831459.1 metallopeptidase family protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 47743.72 Da        Isoelectric Point: 6.2600

>NTDB_id=595032 K3M67_RS10630 WP_285831457.1 2136323..2137690(-) (radA) [Sphingobium sp. V4]
MAKAKRKFVCQQCGTVTSRWLGQCEDCGEWNSIVEEAGETVFSARHDLQNGGRAITLVGLDSAVELPPRTSTGIAEFDRA
LGGGIVTGSATLIGGDPGIGKSTLLLQAAARIASRGLSVAYISGEEAADQVRLRAQRLGLGNAPVQLASATSVRDILTTL
GEGAPPALLIIDSIQTMHSDLIEGAPGTVSQVRASSQELIKFAKQRGTALILVGHVTKDGSIAGPRVLEHMVDTVLAFEG
ERSHQYRILRAVKNRFGGTDEIGVFAMVAEGLEEVANPSALFLTNRDETVTGATVFPALEGTRPVLVEIQALVVRLSSGA
TPRRAVVGWDSGRLAMILAVLEARCGLSFSTCEVYLNIAGGYRLSDPAADLAVAAALISAMSERPVPADIVLFGEIALSS
EIRPVAHSPLRLREAAKLGFDRAYIPASAADGVKGIAVSGFRTLAQLVDQMLGRG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=595032 K3M67_RS10630 WP_285831457.1 2136323..2137690(-) (radA) [Sphingobium sp. V4]
ATGGCCAAGGCAAAACGCAAATTCGTCTGTCAGCAATGCGGCACCGTCACCAGCCGGTGGCTGGGCCAGTGCGAGGATTG
CGGCGAATGGAACAGCATCGTCGAGGAAGCGGGCGAGACCGTTTTCTCCGCGCGCCATGACCTGCAAAATGGCGGCCGGG
CGATCACCCTGGTTGGCCTCGACAGCGCGGTCGAGCTGCCGCCGCGGACCAGCACGGGCATTGCCGAGTTCGACCGGGCG
CTGGGCGGCGGCATCGTCACCGGGTCCGCGACGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACCCTGCTGCTTCA
GGCGGCAGCGCGCATCGCCTCGCGCGGGCTGTCGGTTGCCTATATCAGCGGCGAAGAAGCGGCCGATCAGGTCCGCCTGC
GCGCTCAGCGCCTCGGCCTTGGCAACGCGCCCGTCCAGCTCGCCAGCGCCACTTCGGTCCGCGACATATTGACGACGCTG
GGGGAGGGGGCGCCGCCCGCCTTGCTGATCATCGATTCGATCCAGACGATGCACAGCGACCTGATCGAGGGCGCGCCCGG
CACCGTCAGCCAGGTCCGCGCCTCATCCCAGGAACTTATCAAATTCGCCAAGCAGCGCGGCACCGCGCTCATCCTCGTCG
GCCATGTCACCAAGGATGGCAGCATCGCGGGCCCGCGCGTGCTGGAACATATGGTCGACACGGTGCTGGCCTTCGAGGGG
GAACGCAGCCACCAGTATCGCATCCTGCGCGCGGTCAAGAACCGCTTTGGCGGCACCGACGAGATCGGCGTTTTCGCCAT
GGTCGCCGAGGGGCTGGAGGAGGTCGCCAACCCGTCCGCCCTGTTCCTCACCAACCGCGACGAGACGGTGACGGGCGCGA
CTGTATTCCCCGCGCTGGAGGGCACTCGCCCGGTGCTGGTGGAAATACAGGCGCTGGTCGTGCGCCTCTCCAGTGGCGCG
ACGCCTCGGCGCGCGGTGGTCGGCTGGGACAGCGGCCGGCTCGCCATGATCCTGGCGGTGCTGGAGGCGCGCTGCGGCCT
CAGCTTCTCCACCTGCGAAGTCTATCTGAACATCGCGGGCGGCTATCGCCTGTCGGACCCGGCGGCCGACCTCGCCGTCG
CGGCGGCGCTTATCTCCGCCATGTCGGAACGGCCGGTCCCCGCCGATATCGTCCTCTTCGGGGAAATTGCCCTGTCGAGC
GAAATCCGCCCCGTCGCCCATTCACCGCTGCGTCTGCGCGAAGCGGCGAAACTGGGCTTCGACCGCGCCTATATTCCGGC
GTCCGCCGCCGACGGGGTGAAGGGAATCGCGGTCAGCGGTTTTCGCACCCTTGCCCAGCTGGTTGACCAGATGCTGGGAC
GCGGATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-427)

AAA domain (AAA_25)

(82-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.052

100

0.488
  radA Streptococcus mitis NCTC 12261

46.827

100

0.47
  radA Streptococcus pneumoniae Rx1

46.608

100

0.468
  radA Streptococcus pneumoniae D39

46.608

100

0.468
  radA Streptococcus pneumoniae R6

46.608

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.608

100

0.468
  radA Streptococcus mitis SK321

46.389

100

0.466