Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K2O51_RS14255 Genome accession   NZ_CP080767
Coordinates   381059..382540 (-) Length   493 a.a.
NCBI ID   WP_220631250.1    Uniprot ID   -
Organism   Cupriavidus pinatubonensis strain HN-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 376059..387540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2O51_RS14225 (K2O51_14225) - 376458..377120 (-) 663 WP_011296539.1 LON peptidase substrate-binding domain-containing protein -
  K2O51_RS14230 (K2O51_14230) rapZ 377234..378118 (-) 885 WP_011296540.1 RNase adapter RapZ -
  K2O51_RS14235 (K2O51_14235) - 378226..378597 (-) 372 WP_011296541.1 PsiF family protein -
  K2O51_RS14240 (K2O51_14240) hprK 378774..379745 (-) 972 WP_011296542.1 HPr(Ser) kinase/phosphatase -
  K2O51_RS14245 (K2O51_14245) ptsN 379820..380275 (-) 456 WP_011296543.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K2O51_RS14250 (K2O51_14250) raiA 380656..381009 (-) 354 WP_011296544.1 ribosome-associated translation inhibitor RaiA -
  K2O51_RS14255 (K2O51_14255) rpoN/rpoN1 381059..382540 (-) 1482 WP_220631250.1 RNA polymerase factor sigma-54 Machinery gene
  K2O51_RS14260 (K2O51_14260) lptB 382642..383442 (-) 801 WP_011296546.1 LPS export ABC transporter ATP-binding protein -
  K2O51_RS14265 (K2O51_14265) lptA 383472..384113 (-) 642 WP_011296547.1 lipopolysaccharide transport periplasmic protein LptA -
  K2O51_RS14270 (K2O51_14270) lptC 384200..384826 (-) 627 WP_011296548.1 LPS export ABC transporter periplasmic protein LptC -
  K2O51_RS14275 (K2O51_14275) - 384852..385835 (-) 984 WP_011296549.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54904.13 Da        Isoelectric Point: 4.9129

>NTDB_id=594827 K2O51_RS14255 WP_220631250.1 381059..382540(-) (rpoN/rpoN1) [Cupriavidus pinatubonensis strain HN-2]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPASAPAEPQ
GNGEANADSARGSDDENYTDSGGDDYGSDWSLDDFARRPQGEDDEKTPMQLRDAEPTLREHLMEQLTPLKISLRDKGLAI
FLIESLDDDGYLSTSLDEICSELPEELEFEVDEVQAMLTLLQSFDPAGVGARNAAECLSLQLRRLQHPQRELALSIVQHH
LELLAVRDYTRLKKALQVDEAALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSSWIAQLNPDVMPRLRINDMY
AQILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRSPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKTL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=594827 K2O51_RS14255 WP_220631250.1 381059..382540(-) (rpoN/rpoN1) [Cupriavidus pinatubonensis strain HN-2]
ATGAAACCGTCGCTCCAGCTCCGCCTTTCGCAGCATCTGGCACTCACGCCGCAACTGCAGCAGTCGATCCGACTGCTGCA
ACTCTCAACGCTGGAACTGCAACAGGAAGTCGAGCAGGCGCTCACGGAGAACCCGCTGCTGGAGCGCGAAAACGACTGGA
TCGAAAGTCCGCTGCGCGTAGCGGCAGATGGCTCGGTCAACCTGCAAAGTGCCCCGGCTTCCGCTCCTGCCGAACCGCAG
GGCAATGGCGAAGCGAATGCCGACAGCGCACGCGGCAGCGATGACGAGAACTACACCGACAGCGGCGGTGACGACTACGG
CAGCGACTGGAGCCTCGACGATTTCGCGCGGCGCCCCCAGGGCGAGGACGACGAAAAGACGCCCATGCAGCTGCGCGATG
CCGAGCCCACGCTGCGCGAACATCTGATGGAACAGCTCACGCCGCTGAAGATTTCGCTGCGCGACAAGGGGCTGGCGATC
TTCCTGATCGAATCGCTCGATGACGACGGCTACCTGAGCACGTCGCTCGACGAGATCTGCTCGGAACTGCCCGAGGAACT
CGAATTCGAAGTCGACGAAGTCCAGGCCATGCTCACGCTGCTGCAGAGCTTCGACCCGGCGGGCGTGGGCGCGCGCAATG
CAGCCGAATGCCTGAGCCTGCAATTGCGCCGGCTGCAGCACCCGCAGCGCGAACTGGCGCTGTCGATCGTCCAGCATCAT
CTTGAATTGCTTGCGGTGCGCGACTACACGCGCCTGAAAAAAGCGCTGCAGGTGGACGAAGCGGCGCTAAAAGCCGCGCA
TGAACTGATCCGTTCGCTCGCGCCGTATCCCGGGCATGCGTATAGCCGGCCTGAAGCGGATTTCGTAGTGCCCGACGTGT
TCGTGCGCAAGAGCGGCAGCAGCTGGATTGCACAGCTGAACCCGGATGTGATGCCCCGCCTGCGCATCAACGACATGTAC
GCGCAGATCCTGCGCGGAGCGAAGGGGGAATCCGGCGCTGCCGGGCTGCAGCAAAAGCTGCAGGAGGCACGTTGGCTGAT
CAAAAATATTCAGCAAAGGTTCGACACAATCCTGCGAGTTTCACAGGCAATTGTCGAACGTCAGAAGAACTTTTTCACGC
ACGGCGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGATTACACGAGTCAACCATATCCCGG
GTGACGACCAATAAATACATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGTAGCCACGTGTCCACCGA
AACCGGTGGCGCGGCTTCATCAACGGCGATCCGCGCCTTGATCAAGCAACTTATAGGAGCCGAAGACCCGAGGAGTCCTC
TTTCCGACAGCCGAATTGCCGAATTGCTGGGTGAACAAGGCTTCGTTGTCGCGCGCCGTACCGTCGCCAAGTATCGCGAA
GCCCTGAAGATCCCCGCTGTCAATCTCCGCAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.641

100

0.809