Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K2L43_RS04190 Genome accession   NZ_CP080761
Coordinates   869883..871616 (-) Length   577 a.a.
NCBI ID   WP_012655751.1    Uniprot ID   A0A6M6IAK8
Organism   Diaphorobacter sp. MNS-0     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 864883..876616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2L43_RS04160 (K2L43_04160) - 865542..865988 (+) 447 WP_220638570.1 NUDIX domain-containing protein -
  K2L43_RS04165 (K2L43_04165) - 866057..866287 (-) 231 WP_011804249.1 DNA gyrase inhibitor YacG -
  K2L43_RS04170 (K2L43_04170) zapD 866294..867049 (-) 756 WP_011804250.1 cell division protein ZapD -
  K2L43_RS04175 (K2L43_04175) coaE 867140..867733 (-) 594 WP_253940107.1 dephospho-CoA kinase -
  K2L43_RS04180 (K2L43_04180) - 867765..868646 (-) 882 WP_220638571.1 A24 family peptidase -
  K2L43_RS04185 (K2L43_04185) pilC 868646..869863 (-) 1218 WP_088887536.1 type II secretion system F family protein Machinery gene
  K2L43_RS04190 (K2L43_04190) pilB 869883..871616 (-) 1734 WP_012655751.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K2L43_RS04195 (K2L43_04195) - 871977..873068 (+) 1092 WP_220638572.1 site-specific integrase -
  K2L43_RS04200 (K2L43_04200) - 873310..875739 (-) 2430 WP_135148427.1 DUF262 domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63629.02 Da        Isoelectric Point: 5.3559

>NTDB_id=594783 K2L43_RS04190 WP_012655751.1 869883..871616(-) (pilB) [Diaphorobacter sp. MNS-0]
MAAIDTQKEAQPIALPGLARALMSVGKLDQKAAEEIYKKSQNSRSSFIAELTESKIITPSDLAHTVSTVFGAPLLDVDAI
DRQRIPKDLLDVKLCQSYKVIALSKRNNRLIVATADPTFQEAAEKIKFTTQMGVDWIIAEYDKLLRLIEASTKSGTETLD
GINSSNDFEFGDIPVDEAPDEDADNPAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRIDGELREIASPPI
AIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLQAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPVDIPH
ETLLEAGYRDEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQARLEGVRSLRESGLHK
AKMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=594783 K2L43_RS04190 WP_012655751.1 869883..871616(-) (pilB) [Diaphorobacter sp. MNS-0]
ATGGCTGCTATCGACACACAGAAAGAAGCTCAACCCATTGCCCTACCCGGACTGGCGCGCGCGTTGATGTCTGTGGGCAA
ATTGGACCAAAAAGCGGCGGAAGAGATTTACAAAAAGTCACAAAATAGCCGCAGTAGCTTTATTGCAGAGCTCACGGAAT
CGAAAATCATCACGCCAAGTGATCTGGCCCATACGGTATCCACCGTATTCGGTGCTCCCTTGCTAGATGTCGATGCGATC
GACCGGCAGCGCATCCCCAAAGACCTGTTGGACGTCAAACTTTGCCAGTCCTACAAAGTCATCGCTCTCAGTAAGCGCAA
TAACCGGCTCATCGTCGCCACCGCGGATCCGACATTTCAAGAGGCCGCAGAAAAGATCAAGTTCACCACACAAATGGGGG
TGGACTGGATCATCGCGGAATATGACAAGCTGCTCCGCTTGATTGAGGCGAGCACGAAGAGCGGTACAGAAACGCTCGAC
GGCATTAACAGCAGTAATGATTTTGAGTTCGGCGATATTCCCGTTGATGAAGCTCCGGACGAGGACGCTGACAATCCCGC
GGAAGTCGAGGATGCTCCCGTTGTCAAATTTCTGCACAAGATGCTGCTGGATGCTTTCAATATGCGTGCATCCGATTTGC
ATTTTGAGCCCTACGAGCACCATTACCGTGTCCGCTTCCGCATAGATGGCGAATTGCGCGAAATCGCATCGCCGCCAATT
GCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGATATTTCGGAAAAACGCGTGCCCCAGGATGG
CCGCATGAAGCTCAAGGTTGGGCCGGACCGGGTTATCGATTTCCGCGTCAGCACCCTGCCTACGCTCTTTGGCGAGAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGTATTGACGCACTGGGATATGAGCCCGAGGAAAAGGAGCGT
TTGCTGCAGGCCATCAACCGCCCTTATGGAATGATCCTGGTGACCGGACCCACGGGCTCCGGGAAGACCGTGTCGCTCTA
CACATGTCTCAATTTGTTGAACAAGCCCGGCGTCAATATTGCGACCGCTGAGGATCCCTCGGAAATCAACCTCCCGGGTG
TGAACCAAGTCAACGTCAACGAAAAAGCCGGCCTCACGTTTGCCGTCGCGCTCAAGTCATTCCTTCGCCAAGATCCGGAC
ATCATCATGGTGGGTGAAATCCGGGATCTGGAAACGGCAGACATTTCGATCAAAGCCGCGCAGACGGGTCACTTGGTGCT
GTCCACCCTGCACACCAATGACGCCCCCACGACATTGACGCGCATGCGCAATATGGGTATCGCGCCCTTCAATATCGCAT
CCAGCGTGATTCTTATTACGGCGCAGCGACTGGCGCGTCGCTTGTGCCCCAATTGCAAGGCGCCCGTGGACATTCCACAT
GAGACGCTGCTGGAGGCCGGCTACCGGGACGAGGAGATCGACGGCTCATGGGTCACCTACCGACCGGTGGGCTGCTCCGC
CTGCAATAACGGATACAAGGGGCGCGTGGGCATTTACCAGGTCATGCCGGTGTCCGAAGAAATCCAGCGCATCATCCTGC
GCGATGGAAGCGCACTGGAAATCGCCGAACAGGCACGCCTGGAAGGCGTCCGTTCTCTGCGTGAATCCGGGCTTCACAAG
GCAAAAATGGGATTGACCTCCTTGGAAGAGGTCTTGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M6IAK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.181

99.48

0.539
  pilB Acinetobacter baumannii D1279779

53.723

97.747

0.525
  pilF Neisseria gonorrhoeae MS11

52.389

97.92

0.513
  pilB Legionella pneumophila strain ERS1305867

49.912

97.92

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

45.714

97.054

0.444
  pilB Vibrio campbellii strain DS40M4

45.455

97.227

0.442
  pilB Vibrio cholerae strain A1552

46.881

91.681

0.43
  pilF Thermus thermophilus HB27

39.897

100

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

92.721

0.381