Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K2L43_RS01920 Genome accession   NZ_CP080761
Coordinates   394572..396149 (+) Length   525 a.a.
NCBI ID   WP_220638392.1    Uniprot ID   -
Organism   Diaphorobacter sp. MNS-0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389572..401149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2L43_RS01895 (K2L43_01895) argS 389859..391562 (+) 1704 WP_220638390.1 arginine--tRNA ligase -
  K2L43_RS01900 (K2L43_01900) - 391575..392201 (+) 627 WP_220638391.1 SPOR domain-containing protein -
  K2L43_RS01905 (K2L43_01905) - 392260..392910 (+) 651 WP_011803808.1 thiol:disulfide interchange protein DsbA/DsbL -
  K2L43_RS01910 (K2L43_01910) lptA 393084..393743 (+) 660 WP_041838776.1 lipopolysaccharide transport periplasmic protein LptA -
  K2L43_RS01915 (K2L43_01915) lptB 393767..394519 (+) 753 WP_012655406.1 LPS export ABC transporter ATP-binding protein -
  K2L43_RS01920 (K2L43_01920) rpoN/rpoN1 394572..396149 (+) 1578 WP_220638392.1 RNA polymerase factor sigma-54 Machinery gene
  K2L43_RS01925 (K2L43_01925) - 396153..396596 (+) 444 WP_012655408.1 thioesterase family protein -
  K2L43_RS01930 (K2L43_01930) - 396593..397906 (+) 1314 WP_220638393.1 class I SAM-dependent RNA methyltransferase -
  K2L43_RS01935 (K2L43_01935) - 397896..398381 (+) 486 WP_220638394.1 putative toxin-antitoxin system toxin component, PIN family -
  K2L43_RS01940 (K2L43_01940) - 398369..399670 (+) 1302 WP_220638395.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  K2L43_RS01945 (K2L43_01945) - 400153..400983 (+) 831 WP_220638396.1 YecA family protein -

Sequence

Protein


Download         Length: 525 a.a.        Molecular weight: 57449.53 Da        Isoelectric Point: 4.5772

>NTDB_id=594778 K2L43_RS01920 WP_220638392.1 394572..396149(+) (rpoN/rpoN1) [Diaphorobacter sp. MNS-0]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREEFGLAQADAPVSEQDREAEDAIY
SGAVSAGQESATSQNDAETSTAETTAEPADWEGDGSVDLAPDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFL
HRQALALRLSELDMAALRFLIESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
LTLQLRAMDQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIARLEPRPGRRFADVDRNIIVP
DVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGGRGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQ
KNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1578 bp        

>NTDB_id=594778 K2L43_RS01920 WP_220638392.1 394572..396149(+) (rpoN/rpoN1) [Diaphorobacter sp. MNS-0]
ATGAAGCCCGGCCTGTCGCTGCGCGTCTCGCAGCACCTGGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGCCTGCTGCA
GCTGTCCACGCTGGAGCTGGCCCAGGAGGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCACGCTGGAAGAGG
CCCCGCGCGAGGAGTTCGGCCTGGCCCAGGCTGATGCGCCGGTGAGCGAGCAAGACCGCGAAGCCGAGGATGCTATTTAT
TCAGGAGCTGTCAGCGCAGGACAGGAAAGCGCTACAAGCCAAAATGATGCAGAAACCTCCACCGCCGAAACGACGGCCGA
ACCCGCGGACTGGGAAGGCGACGGCTCGGTAGACCTGGCGCCCGACGACAGCGAGTGGGGCGGCGACGCCCCGGCGCGCA
ACGGTGCCGGCGCGGGGAGCGACGACGAGGTGGACGCCACCGAGCTGGCCCGCAGCCAGGAGTCGCTCACGGCCTTCCTG
CACCGCCAGGCGCTGGCGCTGCGCCTGTCCGAGCTGGACATGGCCGCGCTGCGCTTCCTCATCGAATCGCTGAACGACGA
CGGCTACCTGGACGAGTCGCTGCAGGACCTGGCCGCCAGCCTGGCGGGCACCGAAGACCCCGAGCAACTCGATGAATTGG
TGCACCGCTTCACCGTGGCGCTGCGCCTGCTGCAAAGCCTGGAGCCCGCGGGCGTGGGCGCGCGCGGACTGGCCGAATGC
CTCACGCTGCAGTTGCGTGCGATGGACCAGGACGAAGAGGGCGACCCCGACGTGATCCAGGCCGCGCTGGCCATCTGCGG
CCAGCCGCTGGACCTGCTGGCCAAGCGCGACGTGCGCCGCCTGGCGCAGGCCTGCGGCACCAGCGAAGAGCGCACGCGCG
CCGCGATGGCCTTGATCGCGCGGCTGGAGCCACGCCCGGGCAGGCGCTTTGCCGACGTGGACCGCAACATCATCGTGCCC
GACGTGATCGTCAAGAAAACCGGCCGCGGCGGACAGCAGAACTTCAGCGTGCAGCTCAACCCGGACGTGATGCCGCGCCT
GCGCGTGCACGACATCTACGCCGGTGCGTTGCGCGGCGGCCGTGGCGAAGGCCACCAGGCGCTGCAGGCACGGCTGCAGG
AGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTTGACACCATCCTGCGCGTGTCGCGCGCCATCGTGGAGCGGCAG
AAGAACTTCTTCGTGCATGGCGAACTGGCCATGCGCCCGCTGGTGCTGCGCGACATTGCCGATGAACTGGGCCTGCACGA
GTCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACGCCGCAAGGCACCTACGAACTCAAGTATTTCTTCGGCT
CGGGGCTGGGCACCGAAACCGGCGGCAACGCCTCCAGCACGGCCGTGCGCGCGCTCATCAAGCAGTTCATCGCGGCCGAG
AACCCGCACAAGCCGCTGTCGGACAGCCAGATCGCCGAGATGCTCAAGGAGCAGGGCATTGAATGTGCGCGCCGCACCGT
GGCCAAATACCGTGAGGCGCTCAAGATCGCCCCGGCCAACCTCCGCAAGGCACTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(367-523)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(147-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.471

100

0.526