Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEKA_RS02835 Genome accession   NZ_CP080636
Coordinates   280940..281740 (-) Length   266 a.a.
NCBI ID   WP_005261820.1    Uniprot ID   A0A6N1MKN9
Organism   Acinetobacter lwoffii strain EK30A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275940..286740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEKA_RS02815 (ABEKA_0270) - 276985..277683 (-) 699 WP_004278352.1 tetratricopeptide repeat protein -
  ABEKA_RS02820 (ABEKA_0271) hisS 277714..279009 (-) 1296 WP_005261825.1 histidine--tRNA ligase -
  ABEKA_RS02825 (ABEKA_0272) ispG 279013..280134 (-) 1122 WP_004278355.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEKA_RS02830 (ABEKA_0273) - 280158..280949 (-) 792 WP_004647463.1 DUF4115 domain-containing protein -
  ABEKA_RS02835 (ABEKA_0274) pilF 280940..281740 (-) 801 WP_005261820.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEKA_RS02840 (ABEKA_0275) rlmN 281755..282990 (-) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEKA_RS02845 (ABEKA_0276) ndk 283113..283544 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  ABEKA_RS02850 (ABEKA_0277) iscX 283655..283855 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  ABEKA_RS02855 (ABEKA_0278) - 283943..284494 (-) 552 WP_222110536.1 phosphatase PAP2 family protein -
  ABEKA_RS02860 (ABEKA_0279) - 284515..285795 (-) 1281 WP_121980030.1 glycosyltransferase family 1 protein -
  ABEKA_RS02865 (ABEKA_0280) - 286037..286447 (-) 411 WP_005103244.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30161.32 Da        Isoelectric Point: 9.2878

>NTDB_id=594607 ABEKA_RS02835 WP_005261820.1 280940..281740(-) (pilF) [Acinetobacter lwoffii strain EK30A]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALETTPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWIGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=594607 ABEKA_RS02835 WP_005261820.1 280940..281740(-) (pilF) [Acinetobacter lwoffii strain EK30A]
TTGAGAAATCTAACATCAAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCCAAACGTGCGCTGGATCAGGCACTTGAGACTACTCCGCGTGATTCTCAAGCAAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCAATCGAGCAGTTTCAAATTG
CTGGTGCCACTTTAGGTTATGATCAACGTTTTCGTGCATTGGAAAATATGGGACGGATTTACCTGAAACTGGGTGATACA
GCGAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCTGCGACACAAATGTATCAACAGTTTGTACGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCACTCTGGATTGGCATTCGCACTGCGCGTGCCGGAGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_14)

(40-79)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N1MKN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

97.744

0.771
  pilF Acinetobacter baylyi ADP1

69.925

100

0.699