Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABVS_RS09645 Genome accession   NZ_CP080576
Coordinates   2019187..2019987 (+) Length   266 a.a.
NCBI ID   WP_005103248.1    Uniprot ID   N9QCA1
Organism   Acinetobacter lwoffii strain VS15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2014187..2024987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVS_RS09615 (ABVS_2010) - 2014480..2014890 (+) 411 WP_005103244.1 hypothetical protein -
  ABVS_RS09620 (ABVS_2011) - 2015132..2016412 (+) 1281 WP_222111937.1 glycosyltransferase family 4 protein -
  ABVS_RS09625 (ABVS_2012) - 2016433..2016984 (+) 552 WP_004278361.1 phosphatase PAP2 family protein -
  ABVS_RS09630 (ABVS_2013) iscX 2017072..2017272 (+) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  ABVS_RS09635 (ABVS_2014) ndk 2017383..2017814 (+) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  ABVS_RS09640 (ABVS_2015) rlmN 2017937..2019172 (+) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABVS_RS09645 (ABVS_2016) pilF 2019187..2019987 (+) 801 WP_005103248.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABVS_RS09650 (ABVS_2017) - 2019978..2020769 (+) 792 WP_004647463.1 helix-turn-helix domain-containing protein -
  ABVS_RS09655 (ABVS_2018) ispG 2020793..2021914 (+) 1122 WP_004278355.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABVS_RS09660 (ABVS_2019) hisS 2021918..2023213 (+) 1296 WP_005103249.1 histidine--tRNA ligase -
  ABVS_RS09665 (ABVS_2020) - 2023244..2023942 (+) 699 WP_005103250.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30119.24 Da        Isoelectric Point: 9.2878

>NTDB_id=594203 ABVS_RS09645 WP_005103248.1 2019187..2019987(+) (pilF) [Acinetobacter lwoffii strain VS15]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=594203 ABVS_RS09645 WP_005103248.1 2019187..2019987(+) (pilF) [Acinetobacter lwoffii strain VS15]
TTGAGAAATCTAACATCTAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATTTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
TTGCAGCAGGAAGGTAGCAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCACAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGTGCATTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCGGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTCGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QCA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

97.744

0.767
  pilF Acinetobacter baylyi ADP1

69.549

100

0.695