Detailed information NTG0594050

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: JMO16_RS00270
• Genome accession: NZ_CP080566
• Coordinates: 58922..60271 (-)
• Length: 449 a.a.
• NCBI ID: WP_000815646.1
• Uniprot ID: Q9XB96
• Organism: Staphylococcus aureus strain HL20835 isolate HL20835
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	39009..60271	58922..60271	within	0

Gene organization within MGE regions

Location: 39009..60271

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JMO16_RS00165 (JMO16_00165)	mecA	39009..41018 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
JMO16_RS00170 (JMO16_00170)	mecR1	41115..42872 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
JMO16_RS00175 (JMO16_00175)	mecI	42872..43243 (+)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
JMO16_RS00180 (JMO16_00180)	psm-mec	43328..43396 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
JMO16_RS00185 (JMO16_00185)	-	43730..44860 (+)	1131	WP_010922808.1	ROK family transcriptional regulator	-
JMO16_RS00190 (JMO16_00190)	cstB	44974..46308 (-)	1335	Protein_35	persulfide dioxygenase-sulfurtransferase CstB	-
JMO16_RS00195 (JMO16_00195)	-	46341..47405 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
JMO16_RS00200 (JMO16_00200)	cstR	47541..47801 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
JMO16_RS00205 (JMO16_00205)	-	47801..48439 (+)	639	Protein_38	sulfite exporter TauE/SafE family protein	-
JMO16_RS15015	-	48493..48552 (-)	60	Protein_39	DNA repair protein RadC	-
JMO16_RS00215 (JMO16_00215)	-	48784..49446 (-)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
JMO16_RS00220 (JMO16_00220)	erm(A)	49877..50608 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
JMO16_RS00225 (JMO16_00225)	-	50734..51516 (-)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
JMO16_RS00230 (JMO16_00230)	-	51667..52044 (-)	378	WP_000361059.1	DUF6262 family protein	-
JMO16_RS00235 (JMO16_00235)	-	52051..53943 (-)	1893	WP_001557544.1	site-specific integrase	-
JMO16_RS00240 (JMO16_00240)	-	53940..55025 (-)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
JMO16_RS00245 (JMO16_00245)	-	55144..55461 (-)	318	WP_061641403.1	JAB domain-containing protein	-
JMO16_RS00250 (JMO16_00250)	-	55482..55988 (-)	507	WP_001092169.1	DUF1643 domain-containing protein	-
JMO16_RS00255 (JMO16_00255)	-	56006..56317 (-)	312	WP_000700853.1	DUF960 family protein	-
JMO16_RS00260 (JMO16_00260)	-	56404..56754 (-)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
JMO16_RS00265 (JMO16_00265)	ccrB	57272..58900 (-)	1629	WP_001186602.1	cassette chromosome recombinase CcrB	Machinery gene
JMO16_RS00270 (JMO16_00270)	ccrA	58922..60271 (-)	1350	WP_000815646.1	cassette chromosome recombinase CcrA	Machinery gene

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52607.20 Da
• Isoelectric Point: 9.9585

>NTDB_id=594050 JMO16_RS00270 WP_000815646.1 58922..60271(-) (ccrA) [Staphylococcus aureus strain HL20835 isolate HL20835]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=594050 JMO16_RS00270 WP_000815646.1 58922..60271(-) (ccrA) [Staphylococcus aureus strain HL20835 isolate HL20835]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-322)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9XB96

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	100	100	1
ccrA	Staphylococcus aureus COL	74.833	100	0.748