Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M4R69_RS13455 Genome accession   NZ_CP097198
Coordinates   2772823..2773263 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2767823..2778263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4R69_RS13435 (M4R69_13430) aroP 2768821..2770194 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M4R69_RS13440 (M4R69_13435) ampE 2770237..2771091 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  M4R69_RS13445 (M4R69_13440) ampD 2771088..2771639 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M4R69_RS13450 (M4R69_13445) nadC 2771727..2772620 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M4R69_RS13455 (M4R69_13450) pilA 2772823..2773263 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  M4R69_RS13460 (M4R69_13455) pilB 2773273..2774658 (+) 1386 WP_001025171.1 type II secretion system protein GspE Machinery gene
  M4R69_RS13465 (M4R69_13460) hofC 2774648..2775850 (+) 1203 WP_000157229.1 protein transport protein HofC -
  M4R69_RS13470 (M4R69_13465) guaC 2775885..2776928 (-) 1044 WP_001217338.1 GMP reductase -
  M4R69_RS13475 (M4R69_13470) - 2777084..2777128 (-) 45 WP_120795372.1 protein YacM -
  M4R69_RS13480 (M4R69_13475) coaE 2777153..2777773 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=593932 M4R69_RS13455 WP_000360895.1 2772823..2773263(+) (pilA) [Escherichia coli strain 2-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=593932 M4R69_RS13455 WP_000360895.1 2772823..2773263(+) (pilA) [Escherichia coli strain 2-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363