Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M4R35_RS18900 Genome accession   NZ_CP097181
Coordinates   3850589..3851029 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SH21PTE31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3845589..3856029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4R35_RS18880 aroP 3846587..3847960 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M4R35_RS18885 ampE 3848003..3848857 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  M4R35_RS18890 ampD 3848854..3849405 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M4R35_RS18895 nadC 3849493..3850386 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M4R35_RS18900 pilA 3850589..3851029 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  M4R35_RS18905 pilB 3851039..3852424 (+) 1386 WP_021570596.1 type II secretion system protein GspE Machinery gene
  M4R35_RS18910 hofC 3852414..3853616 (+) 1203 WP_000157266.1 protein transport protein HofC -
  M4R35_RS18915 guaC 3853651..3854694 (-) 1044 WP_001217338.1 GMP reductase -
  M4R35_RS18920 - 3854850..3854894 (-) 45 WP_120795372.1 protein YacM -
  M4R35_RS18925 coaE 3854919..3855539 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=593880 M4R35_RS18900 WP_000360904.1 3850589..3851029(+) (pilA) [Escherichia coli strain SH21PTE31]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=593880 M4R35_RS18900 WP_000360904.1 3850589..3851029(+) (pilA) [Escherichia coli strain SH21PTE31]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363