Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M2M89_RS08500 Genome accession   NZ_CP097130
Coordinates   1641653..1642642 (+) Length   329 a.a.
NCBI ID   WP_003232026.1    Uniprot ID   P51835
Organism   Bacillus subtilis strain S16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1636653..1647642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2M89_RS08485 acpP 1636850..1637083 (+) 234 WP_003154310.1 acyl carrier protein -
  M2M89_RS08490 rncS 1637223..1637972 (+) 750 WP_003232030.1 ribonuclease III -
  M2M89_RS08495 smc 1638073..1641633 (+) 3561 WP_128441627.1 chromosome segregation protein SMC -
  M2M89_RS08500 pilA 1641653..1642642 (+) 990 WP_003232026.1 signal recognition particle-docking protein FtsY Machinery gene
  M2M89_RS08505 ylqB 1642678..1643163 (-) 486 WP_041850836.1 Ig-like domain-containing protein -
  M2M89_RS08510 ylxM 1643341..1643673 (+) 333 WP_003238590.1 putative DNA-binding protein -
  M2M89_RS08515 ffh 1643687..1645027 (+) 1341 WP_014113771.1 signal recognition particle protein -
  M2M89_RS08520 rpsP 1645133..1645405 (+) 273 WP_003220815.1 30S ribosomal protein S16 -
  M2M89_RS08525 ylqC 1645405..1645650 (+) 246 WP_041850835.1 KH domain-containing protein -
  M2M89_RS08530 ylqD 1645772..1646158 (+) 387 WP_014476793.1 YlqD family protein -
  M2M89_RS08535 rimM 1646163..1646687 (+) 525 WP_041850834.1 ribosome maturation factor RimM -
  M2M89_RS08540 trmD 1646684..1647415 (+) 732 WP_003232011.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 36342.52 Da        Isoelectric Point: 5.0164

>NTDB_id=593657 M2M89_RS08500 WP_003232026.1 1641653..1642642(+) (pilA) [Bacillus subtilis strain S16]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide


Download         Length: 990 bp        

>NTDB_id=593657 M2M89_RS08500 WP_003232026.1 1641653..1642642(+) (pilA) [Bacillus subtilis strain S16]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAACTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCCCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAACTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCCGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGTCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAGTTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAGGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGATCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(121-320)

SRP54-type protein, helical bundle domain (SRP54_N)

(34-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P51835

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

43.709

91.793

0.401