Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K1Y48_RS03025 Genome accession   NZ_CP080536
Coordinates   598195..599553 (-) Length   452 a.a.
NCBI ID   WP_215849220.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain GT2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 593195..604553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1Y48_RS03005 (K1Y48_02990) - 594936..595241 (-) 306 WP_064219185.1 Lpp/OprI family alanine-zipper lipoprotein -
  K1Y48_RS03010 (K1Y48_02995) - 595644..596216 (+) 573 WP_215849223.1 hypothetical protein -
  K1Y48_RS03015 (K1Y48_03000) pepP 596225..597571 (-) 1347 WP_215849222.1 Xaa-Pro aminopeptidase -
  K1Y48_RS03020 (K1Y48_03005) - 597587..598024 (-) 438 WP_215849221.1 DUF4168 domain-containing protein -
  K1Y48_RS03025 (K1Y48_03010) radA 598195..599553 (-) 1359 WP_215849220.1 DNA repair protein RadA Machinery gene
  K1Y48_RS03030 (K1Y48_03015) alr 599550..600644 (-) 1095 WP_215849219.1 alanine racemase -
  K1Y48_RS03035 (K1Y48_03020) dnaB 600707..602080 (-) 1374 WP_215849218.1 replicative DNA helicase -
  K1Y48_RS03040 (K1Y48_03025) rplI 602106..602552 (-) 447 WP_064219192.1 50S ribosomal protein L9 -
  K1Y48_RS03045 (K1Y48_03030) - 602590..603507 (-) 918 WP_215849217.1 DUF2232 domain-containing protein -
  K1Y48_RS03050 (K1Y48_03035) rpsR 603523..603801 (-) 279 WP_014027827.1 30S ribosomal protein S18 -
  K1Y48_RS03055 (K1Y48_03040) rpsF 603817..604221 (-) 405 WP_064219194.1 30S ribosomal protein S6 -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 47870.81 Da        Isoelectric Point: 8.3635

>NTDB_id=593609 K1Y48_RS03025 WP_215849220.1 598195..599553(-) (radA) [Acidithiobacillus ferriphilus strain GT2]
MSRDKTLFVCQECGSTSNKWLGRCPDCGAWNSFVEQRMEKTGAKSQTTYATASPPQFLHAVPIADVGRSTTGLAELDRVL
GGGQVPGAAILLGGEPGIGKSTLLLQTAHHLAQASKVLYVTGEESAAQVALRAQRLGLSQSSVRVVAENHLEAIEGLLQS
ERSALVLVDSIQTVYTDTLQSAPGSVAQVRECAARLVRFAKATGTTVWLVGHVTKEGAIAGPRVLEHMVDTVLYFEGEAG
SPYRIVRAIKNRFGAANELGVFQMHEEGLSEVANPSQLFLSQHDRPVAGSVVLATQEGTRPLLVEVQALVTPSPLANPRR
VAIGLDPNRLSLLLAILHRHGGSVFFDQDVFVNIAGGIKVNEPAADLAVALALLSSFRNKPLEGRRVVFGELGLAGEVRA
VAGTEARVREAIKLGFKGAILPRGDKISSTATFKLRPAARLGDAITAAFDGG

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=593609 K1Y48_RS03025 WP_215849220.1 598195..599553(-) (radA) [Acidithiobacillus ferriphilus strain GT2]
ATGAGCCGCGACAAAACCCTCTTTGTCTGTCAGGAATGCGGCAGTACCAGCAACAAATGGCTGGGACGCTGCCCGGATTG
TGGCGCCTGGAACAGCTTCGTCGAACAGCGCATGGAGAAGACCGGGGCCAAATCCCAAACCACCTATGCCACGGCCAGTC
CGCCGCAATTTCTGCACGCGGTGCCGATCGCGGATGTGGGGCGCAGCACGACGGGTCTGGCAGAGCTCGACCGGGTGCTG
GGCGGTGGGCAGGTGCCGGGGGCCGCCATTCTCCTGGGCGGGGAACCGGGTATCGGCAAATCCACCCTGCTGCTCCAGAC
CGCCCATCATTTGGCCCAGGCCAGTAAGGTGCTTTACGTCACCGGCGAGGAGTCCGCGGCACAAGTCGCCTTGCGCGCAC
AGCGCTTGGGGCTGAGCCAGAGCTCCGTACGGGTAGTCGCCGAAAACCATCTTGAAGCGATCGAGGGTCTGCTCCAGTCA
GAGCGATCCGCACTGGTGCTGGTGGATTCTATCCAGACCGTCTATACCGACACGCTGCAATCAGCCCCTGGATCGGTAGC
GCAAGTGCGGGAGTGCGCGGCCCGTCTGGTGCGTTTCGCCAAAGCTACGGGCACCACGGTCTGGCTGGTCGGCCATGTGA
CCAAGGAGGGGGCCATTGCCGGTCCGCGTGTGCTGGAACACATGGTGGATACGGTCCTTTATTTTGAAGGGGAGGCGGGT
AGCCCCTACCGGATTGTGCGTGCCATCAAAAACCGCTTTGGTGCCGCCAATGAACTAGGTGTTTTTCAGATGCATGAAGA
GGGGTTAAGCGAGGTTGCCAACCCTTCACAGCTCTTTCTGTCGCAGCATGACCGACCCGTAGCAGGGAGCGTGGTGCTGG
CGACGCAGGAGGGAACCCGCCCGCTTCTGGTGGAAGTGCAGGCTCTGGTGACACCGAGTCCGCTGGCTAACCCCCGCAGG
GTCGCCATCGGCCTGGACCCTAACCGCCTTTCCCTCCTGCTGGCTATCCTGCACCGCCACGGCGGCAGTGTGTTTTTTGA
TCAGGACGTTTTTGTGAATATCGCAGGTGGAATCAAAGTCAACGAACCAGCCGCCGATCTTGCGGTGGCCTTGGCCCTGC
TCAGCAGTTTCCGCAACAAACCTTTGGAGGGGCGCCGGGTTGTTTTCGGCGAGCTGGGTCTGGCGGGAGAGGTGCGTGCG
GTGGCGGGCACTGAAGCGCGGGTGCGGGAGGCGATCAAATTGGGTTTTAAGGGTGCGATTCTTCCGCGTGGCGACAAGAT
TTCGAGTACCGCTACTTTCAAGTTGCGTCCGGCTGCCCGTCTTGGCGATGCCATAACAGCGGCCTTCGACGGGGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-423)

AAA domain (AAA_25)

(65-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.054

94.469

0.482
  radA Streptococcus pneumoniae Rx1

48.148

95.575

0.46
  radA Streptococcus pneumoniae D39

48.148

95.575

0.46
  radA Streptococcus pneumoniae R6

48.148

95.575

0.46
  radA Streptococcus pneumoniae TIGR4

48.148

95.575

0.46
  radA Streptococcus mitis SK321

48.148

95.575

0.46
  radA Streptococcus mitis NCTC 12261

48.148

95.575

0.46