Detailed information NTG0593442

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K1T67_RS00845
• Genome accession: NZ_CP080511
• Coordinates: 180524..181672 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain DJ06
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 175524..186672

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K1T67_RS00825 (K1T67_00825)	-	176262..177446 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
K1T67_RS00830 (K1T67_00830)	-	177443..178816 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
K1T67_RS00835 (K1T67_00835)	-	179059..179463 (-)	405	WP_003084560.1	NINE protein	-
K1T67_RS00840 (K1T67_00840)	czcD	179617..180516 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
K1T67_RS00845 (K1T67_00845)	pilU	180524..181672 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
K1T67_RS00850 (K1T67_00850)	pilT	181850..182884 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
K1T67_RS00855 (K1T67_00855)	-	183099..183791 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
K1T67_RS00860 (K1T67_00860)	proC	183803..184624 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
K1T67_RS00865 (K1T67_00865)	-	184635..185228 (+)	594	WP_003084544.1	YggT family protein	-
K1T67_RS00870 (K1T67_00870)	-	185225..185521 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=593442 K1T67_RS00845 WP_003100959.1 180524..181672(-) (pilU) [Pseudomonas aeruginosa strain DJ06]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=593442 K1T67_RS00845 WP_003100959.1 180524..181672(-) (pilU) [Pseudomonas aeruginosa strain DJ06]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382