Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K1T74_RS01430 Genome accession   NZ_CP080462
Coordinates   304174..305190 (+) Length   338 a.a.
NCBI ID   WP_220105968.1    Uniprot ID   -
Organism   Vibrio cholerae strain ICDC-VC702     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299174..310190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1T74_RS01415 - 299714..300445 (-) 732 WP_000495223.1 glutathione peroxidase -
  K1T74_RS01420 oxyR 300585..301478 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  K1T74_RS01425 - 301554..304031 (-) 2478 WP_032470274.1 penicillin-binding protein 1A -
  K1T74_RS01430 pilM 304174..305190 (+) 1017 WP_220105968.1 type IV pilus assembly protein PilM Machinery gene
  K1T74_RS01435 pilN 305180..305764 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  K1T74_RS01440 pilO 305757..306347 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  K1T74_RS01445 pilP 306337..306855 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  K1T74_RS01450 pilQ 306881..308596 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  K1T74_RS01455 aroK 308783..309307 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37719.49 Da        Isoelectric Point: 7.3328

>NTDB_id=593036 K1T74_RS01430 WP_220105968.1 304174..305190(+) (pilM) [Vibrio cholerae strain ICDC-VC702]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGIRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=593036 K1T74_RS01430 WP_220105968.1 304174..305190(+) (pilM) [Vibrio cholerae strain ICDC-VC702]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCTCGCAGCAGTACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAATTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485