Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M2I95_RS02080 Genome accession   NZ_CP096953
Coordinates   458020..459375 (+) Length   451 a.a.
NCBI ID   WP_126450847.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY5535     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453020..464375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I95_RS02060 (M2I95_02060) - 453857..454423 (-) 567 WP_003084491.1 hypothetical protein -
  M2I95_RS02065 (M2I95_02065) rsmD 454426..455022 (-) 597 WP_221345477.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  M2I95_RS02070 (M2I95_02070) - 455022..456509 (-) 1488 WP_221345478.1 pitrilysin family protein -
  M2I95_RS02075 (M2I95_02075) - 456502..457899 (-) 1398 WP_003084498.1 pitrilysin family protein -
  M2I95_RS02080 (M2I95_02080) pilA 458020..459375 (+) 1356 WP_126450847.1 signal recognition particle-docking protein FtsY Machinery gene
  M2I95_RS02085 (M2I95_02085) ftsE 459372..460043 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  M2I95_RS02090 (M2I95_02090) ftsX 460043..461050 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  M2I95_RS02095 (M2I95_02095) rpoH 461164..462018 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  M2I95_RS02100 (M2I95_02100) - 462083..462688 (+) 606 WP_003084511.1 hypothetical protein -
  M2I95_RS02105 (M2I95_02105) mtgA 462693..463424 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  M2I95_RS02110 (M2I95_02110) - 463461..463838 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  M2I95_RS02115 (M2I95_02115) thiS 463941..464141 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 47980.41 Da        Isoelectric Point: 4.5553

>NTDB_id=592559 M2I95_RS02080 WP_126450847.1 458020..459375(+) (pilA) [Pseudomonas aeruginosa strain NY5535]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPPAPVAEA
PLAGDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASI
GEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVD
VAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAV
QAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKL
DGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=592559 M2I95_RS02080 WP_126450847.1 458020..459375(+) (pilA) [Pseudomonas aeruginosa strain NY5535]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCGATGACGTGGCGC
AATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGCCGGCGCCGGTTGCCGAAGCG
CCGCTCGCCGGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGCCCCGAGGCGCCCGTCGCACAACCCGAGCCTAT
CCTCGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTC
CGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGCATC
GGCGAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCT
GACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCG
ACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGAC
GTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAA
GAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGG
TCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTG
CAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGA
GGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCG
GCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTTGCCCTGACCAAACTG
GACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGA
GGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(246-445)

SRP54-type protein, helical bundle domain (SRP54_N)

(161-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

46.667

96.452

0.45