Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KY495_RS06055 Genome accession   NZ_CP080378
Coordinates   1362094..1363566 (-) Length   490 a.a.
NCBI ID   WP_219882818.1    Uniprot ID   -
Organism   Massilia sp. PAMC28688     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1357094..1368566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KY495_RS06040 (KY495_06040) rapZ 1359488..1360351 (+) 864 WP_219884130.1 RNase adapter RapZ -
  KY495_RS06045 (KY495_06045) - 1360391..1361479 (-) 1089 WP_219882816.1 enoyl-CoA hydratase/isomerase family protein -
  KY495_RS06050 (KY495_06050) hpf 1361649..1362002 (-) 354 WP_219882817.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KY495_RS06055 (KY495_06055) rpoN/rpoN1 1362094..1363566 (-) 1473 WP_219882818.1 RNA polymerase factor sigma-54 Machinery gene
  KY495_RS06060 (KY495_06060) lptB 1363570..1364325 (-) 756 WP_219882819.1 LPS export ABC transporter ATP-binding protein -
  KY495_RS06065 (KY495_06065) lptA 1364328..1364933 (-) 606 WP_219882820.1 lipopolysaccharide transport periplasmic protein LptA -
  KY495_RS06070 (KY495_06070) lptC 1364970..1365563 (-) 594 WP_219882821.1 LPS export ABC transporter periplasmic protein LptC -
  KY495_RS06075 (KY495_06075) - 1365563..1366075 (-) 513 WP_219884131.1 HAD family hydrolase -
  KY495_RS06080 (KY495_06080) - 1366129..1367160 (-) 1032 WP_374040991.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 490 a.a.        Molecular weight: 54045.46 Da        Isoelectric Point: 4.8669

>NTDB_id=592407 KY495_RS06055 WP_219882818.1 1362094..1363566(-) (rpoN/rpoN1) [Massilia sp. PAMC28688]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDHSVRLLADGAIGSNATPGEAAPEPG
AQDAPAPAEAEGGDAPDSEAPQGPEGDADWGEQTRAKGSDDEDSRPQLEASSTTLREHLMEQMRVTVSEGRDRALVELII
DALDDNGYLTETLEEIHERLPAELEIDFDEMKTALALLQSFDPVGVGARSASECLCLQIKAMPGVPLVTRRMALAIVDKH
LAWFAQREFNKLKKALDCDDEDLREAQAVIRQCNPHPGAVFASDVSDFVVPDVIVRKAKGGWSVSLNNEVMPRLRVNNMY
ASLLKQGKGEGQMSAQLQEAKWLIKNMRQRFDTILRVSEAIVERQRNFFSHGAVAMRPLVLREIADTLGLHESTISRVTT
QKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREALK
IPPVSLRKCL

Nucleotide


Download         Length: 1473 bp        

>NTDB_id=592407 KY495_RS06055 WP_219882818.1 1362094..1363566(-) (rpoN/rpoN1) [Massilia sp. PAMC28688]
ATGAAACAGTCACTTCAACTACGCACCTCGCAGCACCTGGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAGCTGCACCAGGAACTGGAGCAGCTGCTGACCGACAATCCGCTGCTCGAACGCCTGGACGATCCGC
TTGACCACTCGGTACGCCTGCTGGCCGACGGCGCCATCGGCAGCAATGCCACGCCGGGCGAAGCGGCGCCGGAGCCGGGC
GCCCAGGATGCACCGGCGCCGGCCGAAGCGGAGGGTGGCGATGCGCCGGACAGCGAAGCGCCGCAGGGACCGGAAGGCGA
CGCCGACTGGGGCGAACAGACCCGTGCCAAGGGCTCGGACGATGAAGACTCGCGCCCGCAGCTGGAAGCGTCGTCGACCA
CGCTGCGCGAGCACCTGATGGAGCAGATGCGGGTAACGGTGAGCGAAGGGCGCGACCGCGCACTGGTGGAGCTGATCATC
GATGCGCTCGACGACAATGGCTACCTGACCGAAACGCTGGAAGAGATCCACGAACGCCTGCCGGCCGAACTGGAGATCGA
CTTCGACGAAATGAAGACCGCGCTGGCGCTGCTGCAAAGCTTTGACCCGGTGGGCGTGGGGGCGCGTTCGGCCTCGGAGT
GCCTGTGCCTGCAGATCAAGGCCATGCCGGGCGTACCGCTGGTCACGCGGCGCATGGCCCTGGCCATCGTCGACAAGCAC
CTGGCCTGGTTCGCGCAGCGCGAATTCAACAAGCTGAAAAAGGCCCTCGACTGCGATGACGAAGACCTGCGCGAGGCGCA
GGCCGTGATCCGCCAGTGCAATCCGCATCCGGGCGCCGTGTTCGCGTCGGACGTGTCGGACTTCGTGGTGCCGGACGTGA
TCGTGCGCAAGGCCAAGGGCGGCTGGTCGGTATCGCTCAACAATGAAGTCATGCCGCGCCTGCGGGTGAACAATATGTAT
GCCAGCCTGCTCAAGCAGGGCAAGGGCGAAGGACAGATGAGCGCGCAGCTGCAGGAAGCGAAGTGGCTGATCAAAAACAT
GCGCCAGCGTTTCGACACCATCCTGCGTGTTTCGGAGGCAATTGTTGAGCGCCAGCGCAATTTCTTTTCGCACGGGGCGG
TTGCCATGCGCCCACTTGTGCTGCGCGAAATTGCTGATACACTTGGTCTGCACGAGAGCACCATCTCTCGTGTCACCACG
CAGAAATACATGCTGACCCCGCACGGCATGTTTGAGTTGAAGTACTTTTTCGGCAGCCACGTCGCCACCGAAGCGGGGGG
AGAAGCATCCTCGACGGCGATTCGCGCGCTGATTGTGCAATTGACAGGAGCAGAAGACCCTAAAAATCCATTATCCGACA
GTAAGATTGCGGACATGCTGGGGGAACAGGGTATGGTCATTGCGCGCCGGACCGTTGCCAAATACCGGGAAGCGCTCAAA
ATCCCGCCCGTCAGTCTCCGAAAGTGTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-320)

Sigma-54, DNA binding domain (Sigma54_DBD)

(333-488)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.01

100

0.608