Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KZW87_RS08450 Genome accession   NZ_CP080222
Coordinates   1691379..1692353 (-) Length   324 a.a.
NCBI ID   WP_031587486.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NT_8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1686379..1697353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZW87_RS08410 (KZW87_08380) gcvT 1686880..1687971 (-) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -
  KZW87_RS08415 (KZW87_08385) - 1688130..1688654 (-) 525 WP_001015120.1 shikimate kinase -
  KZW87_RS08420 (KZW87_08390) - 1688644..1688790 (-) 147 WP_001792109.1 hypothetical protein -
  KZW87_RS08425 (KZW87_08395) comGF 1688887..1689384 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  KZW87_RS08430 (KZW87_08400) comGE 1689302..1689601 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  KZW87_RS08435 (KZW87_08405) comGD 1689588..1690034 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KZW87_RS08440 (KZW87_08410) comGC 1690012..1690323 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KZW87_RS08445 (KZW87_08415) comGB 1690337..1691407 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KZW87_RS08450 (KZW87_08420) comGA 1691379..1692353 (-) 975 WP_031587486.1 competence type IV pilus ATPase ComGA Machinery gene
  KZW87_RS08455 (KZW87_08425) - 1692405..1693028 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KZW87_RS08460 (KZW87_08430) - 1693025..1693354 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KZW87_RS08465 (KZW87_08435) - 1693354..1694340 (-) 987 WP_000161314.1 ROK family glucokinase -
  KZW87_RS08470 (KZW87_08440) - 1694337..1694540 (-) 204 WP_000087561.1 YqgQ family protein -
  KZW87_RS08475 (KZW87_08445) - 1694521..1695984 (-) 1464 WP_001017858.1 rhomboid family intramembrane serine protease -
  KZW87_RS08480 (KZW87_08450) - 1695996..1696535 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  KZW87_RS08485 (KZW87_08455) rpmG 1696744..1696893 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36955.99 Da        Isoelectric Point: 8.6529

>NTDB_id=591466 KZW87_RS08450 WP_031587486.1 1691379..1692353(-) (comGA) [Staphylococcus aureus strain NT_8]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKADVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=591466 KZW87_RS08450 WP_031587486.1 1691379..1692353(-) (comGA) [Staphylococcus aureus strain NT_8]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGATGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997