Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M2J77_RS01985 Genome accession   NZ_CP096909
Coordinates   441075..442427 (+) Length   450 a.a.
NCBI ID   WP_003123870.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY8688     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436075..447427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J77_RS01965 (M2J77_01965) - 436912..437478 (-) 567 WP_003084491.1 hypothetical protein -
  M2J77_RS01970 (M2J77_01970) rsmD 437481..438077 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  M2J77_RS01975 (M2J77_01975) - 438077..439564 (-) 1488 WP_003102862.1 M16 family metallopeptidase -
  M2J77_RS01980 (M2J77_01980) - 439557..440954 (-) 1398 WP_003084498.1 M16 family metallopeptidase -
  M2J77_RS01985 (M2J77_01985) pilA 441075..442427 (+) 1353 WP_003123870.1 signal recognition particle-docking protein FtsY Machinery gene
  M2J77_RS01990 (M2J77_01990) ftsE 442424..443095 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  M2J77_RS01995 (M2J77_01995) ftsX 443095..444102 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  M2J77_RS02000 (M2J77_02000) rpoH 444216..445070 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  M2J77_RS02005 (M2J77_02005) - 445135..445740 (+) 606 WP_003084511.1 hypothetical protein -
  M2J77_RS02010 (M2J77_02010) mtgA 445745..446476 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  M2J77_RS02015 (M2J77_02015) - 446513..446890 (+) 378 WP_003121748.1 DUF423 domain-containing protein -
  M2J77_RS02020 (M2J77_02020) thiS 446993..447193 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 47872.27 Da        Isoelectric Point: 4.5795

>NTDB_id=591248 M2J77_RS01985 WP_003123870.1 441075..442427(+) (pilA) [Pseudomonas aeruginosa strain NY8688]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIG
EGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDV
AREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQ
AAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLD
GTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=591248 M2J77_RS01985 WP_003123870.1 441075..442427(+) (pilA) [Pseudomonas aeruginosa strain NY8688]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTCCGG
CAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGCATCGGC
GAAGGCATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGAC
CGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACA
GCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTC
GCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAA
GCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCT
GGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAG
GCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGA
GCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCA
CCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTTGCCCTGACCAAACTGGAC
GGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGG
GATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(245-444)

SRP54-type protein, helical bundle domain (SRP54_N)

(160-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.454

96

0.456