Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KYI13_RS07570 Genome accession   NZ_CP079984
Coordinates   1442309..1443277 (-) Length   322 a.a.
NCBI ID   WP_188017276.1    Uniprot ID   A0AAE7Q585
Organism   Macrococcoides bohemicum strain 19Msa0383     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1437309..1448277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYI13_RS07540 (KYI13_07545) gcvT 1437961..1439046 (-) 1086 WP_203546562.1 glycine cleavage system aminomethyltransferase GcvT -
  KYI13_RS07545 (KYI13_07550) - 1439183..1439689 (-) 507 WP_188017279.1 shikimate kinase -
  KYI13_RS07550 (KYI13_07555) - 1439870..1440316 (-) 447 WP_188017278.1 competence type IV pilus minor pilin ComGF -
  KYI13_RS07555 (KYI13_07560) - 1440496..1440942 (-) 447 WP_219497451.1 hypothetical protein -
  KYI13_RS07560 (KYI13_07565) comGC 1440914..1441273 (-) 360 WP_096076364.1 competence type IV pilus major pilin ComGC Machinery gene
  KYI13_RS07565 (KYI13_07570) comGB 1441285..1442322 (-) 1038 WP_203545537.1 competence type IV pilus assembly protein ComGB -
  KYI13_RS07570 (KYI13_07575) comGA 1442309..1443277 (-) 969 WP_188017276.1 competence type IV pilus ATPase ComGA Machinery gene
  KYI13_RS07575 (KYI13_07580) - 1443487..1443735 (+) 249 WP_099578437.1 DUF2626 domain-containing protein -
  KYI13_RS07580 (KYI13_07585) - 1443761..1444390 (-) 630 WP_133353337.1 MBL fold metallo-hydrolase -
  KYI13_RS07585 (KYI13_07590) - 1444483..1444722 (+) 240 WP_188017275.1 DUF2759 domain-containing protein -
  KYI13_RS07590 (KYI13_07595) - 1444759..1445070 (-) 312 WP_188017274.1 MTH1187 family thiamine-binding protein -
  KYI13_RS07595 (KYI13_07600) - 1445070..1446041 (-) 972 WP_096076357.1 ROK family glucokinase -
  KYI13_RS07600 (KYI13_07605) - 1446038..1446229 (-) 192 WP_096076356.1 YqgQ family protein -
  KYI13_RS07605 (KYI13_07610) - 1446234..1447658 (-) 1425 WP_219497452.1 rhomboid family intramembrane serine protease -
  KYI13_RS07610 (KYI13_07615) - 1447655..1448221 (-) 567 WP_188017272.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 36746.65 Da        Isoelectric Point: 7.5532

>NTDB_id=590614 KYI13_RS07570 WP_188017276.1 1442309..1443277(-) (comGA) [Macrococcoides bohemicum strain 19Msa0383]
MEKLLHTILDDAIAKKTSDVHFIPEVKNVMMKFRIGGNMYAYNQLNTEDFEKLLSYVKFITHLDVSEKNKAQSGILTYQK
DKILYNIRASTLPHTIGQEACVMRIIPALFSQNISIEQELLSLMHKGSGLILISGPTGSGKSTLMYQMLNYAAQNLNKQI
ISIEDPVEQRIHGMIQINVNRKANITYQNAIKAILRCDPDIIMIGEIRDIETAKHVINASLSGHLVLSTLHANTCLGAIH
RLLEMGITKVELEQCVEIIINQRLVNTTSARTRIFEQLIKGDVRDYLNDRKVQHYHPLSEQLQVLYDKEKITQLEYERYA
LE

Nucleotide


Download         Length: 969 bp        

>NTDB_id=590614 KYI13_RS07570 WP_188017276.1 1442309..1443277(-) (comGA) [Macrococcoides bohemicum strain 19Msa0383]
ATGGAAAAACTATTACATACAATATTGGATGATGCAATTGCTAAAAAAACTTCAGATGTACACTTTATACCTGAAGTGAA
GAATGTCATGATGAAATTCAGGATTGGAGGTAATATGTATGCCTATAATCAGTTAAATACCGAAGACTTCGAAAAATTAT
TATCATATGTAAAATTTATAACACATCTTGATGTTTCTGAAAAAAATAAAGCGCAGAGCGGTATACTGACCTATCAGAAG
GACAAAATACTTTATAATATCAGGGCATCTACATTACCACATACGATTGGACAGGAAGCATGTGTGATGCGAATAATTCC
AGCTTTATTTAGTCAAAATATTTCGATTGAACAGGAATTATTATCTTTGATGCATAAAGGAAGCGGATTAATTCTAATTA
GTGGTCCTACTGGAAGCGGCAAAAGTACTTTAATGTACCAAATGTTAAATTATGCTGCACAAAATTTAAATAAACAGATT
ATATCGATTGAAGATCCTGTTGAACAGAGAATTCATGGTATGATTCAAATCAATGTGAATAGAAAAGCTAATATAACATA
TCAGAATGCAATAAAGGCTATTCTTCGCTGTGATCCGGATATTATTATGATAGGAGAGATCCGTGATATAGAAACTGCAA
AACATGTCATCAATGCAAGTTTAAGTGGTCATCTTGTGCTGTCGACACTTCATGCAAATACATGTCTTGGCGCTATCCAT
CGATTGCTGGAAATGGGAATAACAAAAGTTGAACTTGAACAGTGTGTAGAAATAATCATTAATCAGCGATTAGTTAATAC
TACATCAGCGCGAACACGTATTTTTGAACAGTTGATTAAAGGCGATGTACGGGATTATTTAAATGATAGAAAGGTTCAGC
ATTATCATCCTTTATCTGAACAATTGCAAGTTTTGTACGACAAAGAAAAGATTACGCAGCTGGAGTATGAACGTTATGCT
TTGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

53.055

96.584

0.512
  comGA Staphylococcus aureus N315

53.055

96.584

0.512