Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KYI09_RS10550 Genome accession   NZ_CP079965
Coordinates   2001220..2002581 (-) Length   453 a.a.
NCBI ID   WP_015912383.1    Uniprot ID   B9E8S3
Organism   Macrococcoides caseolyticum strain 19Msa0687     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1996220..2007581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYI09_RS10525 (KYI09_10530) cysE 1996646..1997299 (-) 654 WP_219522400.1 serine O-acetyltransferase -
  KYI09_RS10530 (KYI09_10535) gltX 1997537..1998991 (-) 1455 WP_101044643.1 glutamate--tRNA ligase -
  KYI09_RS10535 (KYI09_10540) ispF 1999009..1999488 (-) 480 WP_144781518.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  KYI09_RS10540 (KYI09_10545) ispD 1999485..2000165 (-) 681 WP_144781519.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KYI09_RS10545 (KYI09_10550) - 2000158..2001207 (-) 1050 WP_015912382.1 PIN/TRAM domain-containing protein -
  KYI09_RS10550 (KYI09_10555) radA 2001220..2002581 (-) 1362 WP_015912383.1 DNA repair protein RadA Machinery gene
  KYI09_RS10555 (KYI09_10560) - 2002804..2005230 (-) 2427 WP_101141329.1 ATP-dependent Clp protease ATP-binding subunit -
  KYI09_RS10560 (KYI09_10565) - 2005240..2006244 (-) 1005 WP_133446051.1 protein arginine kinase -
  KYI09_RS10565 (KYI09_10570) - 2006234..2006620 (-) 387 WP_015912387.1 UvrB/UvrC motif-containing protein -
  KYI09_RS10570 (KYI09_10575) - 2006631..2007092 (-) 462 WP_101141334.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50240.85 Da        Isoelectric Point: 7.2379

>NTDB_id=590561 KYI09_RS10550 WP_015912383.1 2001220..2002581(-) (radA) [Macrococcoides caseolyticum strain 19Msa0687]
MAKVKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKNAISESQSDNKVEKLKDITKESVPRDHTQMKELDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQNHPVLYISGEESVRQTKLRADRLLEDAGELDVYAETNLQIIHETV
KRSKPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMKNTGLKEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVTPTSFHNP
RRMASGVDHNRLNLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSVIVSIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNINEALKMAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=590561 KYI09_RS10550 WP_015912383.1 2001220..2002581(-) (radA) [Macrococcoides caseolyticum strain 19Msa0687]
TTGGCAAAGGTGAAAAGTACATTTGAATGTATGGCCTGTGGTTATCAGTCACCGAAATGGATGGGAAAATGTCCGAATTG
TGGTGCATGGAATCAAATGGAAGAAGTTATTGAACATAAACAGAAGGGTCCTAAAAATGCGATTTCTGAATCACAAAGTG
ATAATAAAGTTGAAAAACTAAAAGATATAACGAAAGAAAGTGTTCCTCGTGATCACACGCAGATGAAGGAACTAGATCGT
GTACTTGGAGGAGGAATCGTTCCCGGTTCATTGATACTTATCGGCGGAGACCCAGGGATTGGGAAATCCACATTACTACT
ACAAGTTTGTGCAATGCTTTCACAAAACCATCCCGTACTTTATATTTCTGGAGAGGAATCGGTAAGACAAACGAAACTAC
GTGCCGATCGTCTATTAGAGGATGCAGGGGAGCTTGATGTCTATGCAGAAACCAACCTTCAGATTATTCACGAAACGGTA
AAACGATCGAAACCGAAATTTTTAGTTATCGATTCAATTCAAACAATATTTCATCCAGAGGTTACAAGTGCACCTGGTTC
TGTATCGCAAGTTAGAGAATGTACACAAGAGTTAATGCGTATCGCAAAACAGATGAACATTGCAACGTTTATCGTAGGGC
ACGTAACAAAAGAAGGACAAATTGCAGGTCCAAGGCTACTAGAACATATGGTTGATACAGTTCTTTACTTTGAAGGAGAC
ACTCATCACAGCTACAGAATATTACGCGCGGTGAAGAATCGTTTTGGTTCGACAAATGAAATGGGTATATTTGAAATGAA
GAATACAGGCCTTAAAGAAGTGCTGAACCCTTCAGAAATGTTCCTTGAAGAACGTACAAAAAATGTAGCGGGTTCAACTA
TCGTTGCAACGATGGAAGGCACGCGTCCATTACTAGTCGAAGTTCAGTCACTTGTAACGCCGACATCCTTTCATAATCCA
AGAAGAATGGCATCAGGTGTGGATCATAATCGTCTGAATTTACTGATGGCTGTACTAGAGAAAAAACAAGGATATTTATT
ACAGCAGCAGGATGCATATGTTAAGGTTGCGGGCGGTGTCAAACTTGATGAACCTGCTGTTGACTTAAGTGTAATCGTAA
GCATTGCTTCAAGCTATAACGATAAGCCGACACGAGGAGATGACTGTTTTATCGGAGAAGTCGGTCTGACTGGTGAAGTG
AGACGTGTTGCGCGTATTGAACAACGTGTACAAGAAGCTGAAAAACTCGGTTTTAAACGTGTTATCATCCCCAAAAATAA
TATTGGTGGCTGGGATTTCCCTGGAAATATAGAAGTAATCGGTGTAACTAATATTAACGAAGCGTTAAAAATGGCATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9E8S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.447

100

0.669
  radA Streptococcus pneumoniae Rx1

62.996

100

0.631
  radA Streptococcus pneumoniae D39

62.996

100

0.631
  radA Streptococcus pneumoniae R6

62.996

100

0.631
  radA Streptococcus pneumoniae TIGR4

62.996

100

0.631
  radA Streptococcus mitis NCTC 12261

62.996

100

0.631
  radA Streptococcus mitis SK321

62.996

100

0.631