Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EGT72_RS13165 Genome accession   NZ_CP079792
Coordinates   2664133..2664933 (-) Length   266 a.a.
NCBI ID   WP_004697674.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ_082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659133..2669933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT72_RS13145 (EGT72_013145) - 2660201..2660899 (-) 699 WP_125280202.1 tetratricopeptide repeat protein -
  EGT72_RS13150 (EGT72_013150) hisS 2660927..2662222 (-) 1296 WP_125280203.1 histidine--tRNA ligase -
  EGT72_RS13155 (EGT72_013155) ispG 2662226..2663347 (-) 1122 WP_086210453.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EGT72_RS13160 (EGT72_013160) - 2663366..2664142 (-) 777 WP_125280204.1 helix-turn-helix domain-containing protein -
  EGT72_RS13165 (EGT72_013165) pilF 2664133..2664933 (-) 801 WP_004697674.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EGT72_RS13170 (EGT72_013170) rlmN 2664950..2666185 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EGT72_RS13175 (EGT72_013175) ndk 2666308..2666739 (-) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  EGT72_RS13180 (EGT72_013180) iscX 2666850..2667050 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  EGT72_RS13185 (EGT72_013185) - 2667141..2667692 (-) 552 WP_125280205.1 phosphatase PAP2 family protein -
  EGT72_RS13190 (EGT72_013190) - 2667885..2669165 (-) 1281 WP_125280206.1 glycosyltransferase family 1 protein -
  EGT72_RS13195 (EGT72_013195) - 2669306..2669722 (-) 417 WP_004983505.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=589525 EGT72_RS13165 WP_004697674.1 2664133..2664933(-) (pilF) [Acinetobacter johnsonii strain AJ_082]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=589525 EGT72_RS13165 WP_004697674.1 2664133..2664933(-) (pilF) [Acinetobacter johnsonii strain AJ_082]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTATG
ATGCTGCGAAACGTACATTGGATCAAGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCAGAAGCAGAAGCAGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTGGGCGAGAAAAATTTAG
ATGCGCGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647