Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KXZ80_RS00435 Genome accession   NZ_CP079737
Coordinates   50508..51887 (+) Length   459 a.a.
NCBI ID   WP_021431480.1    Uniprot ID   T4VZN9
Organism   Paraclostridium bifermentans strain DSM 14991     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 45508..56887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KXZ80_RS00425 (KXZ80_00425) - 45908..47842 (+) 1935 WP_021431478.1 translation factor GTPase family protein -
  KXZ80_RS00430 (KXZ80_00430) - 47970..50270 (+) 2301 WP_021431479.1 ATP-dependent Clp protease ATP-binding subunit -
  KXZ80_RS00435 (KXZ80_00435) radA 50508..51887 (+) 1380 WP_021431480.1 DNA repair protein RadA Machinery gene
  KXZ80_RS00440 (KXZ80_00440) disA 51889..52959 (+) 1071 WP_021428177.1 DNA integrity scanning diadenylate cyclase DisA -
  KXZ80_RS00445 (KXZ80_00445) - 53058..54146 (+) 1089 WP_021431481.1 PIN/TRAM domain-containing protein -
  KXZ80_RS00450 (KXZ80_00450) ispD 54282..54962 (+) 681 WP_021431482.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KXZ80_RS00455 (KXZ80_00455) ispF 54979..55458 (+) 480 WP_021428178.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50137.08 Da        Isoelectric Point: 8.5410

>NTDB_id=589083 KXZ80_RS00435 WP_021431480.1 50508..51887(+) (radA) [Paraclostridium bifermentans strain DSM 14991]
MAKIKTKYVCQECGYENSKWLGKCPECSKWNTFVEEIEEKTSKSNKEVFVIDKSSSRPLNINSIETIKEQRFSTCINELD
RVLGGGVVKGSLVLVGGDPGIGKSTLLIQVSSNVANSGKKVLYISGEESASQIKMRAQRLGIKSDNLYIFAENNLSIIEA
HLESVNPDLIILDSIQTVFSPEITSAPGTVSQIKEGTSRFMKISKKMGISTFVVGHVTKEGALAGPKVLEHMVDTVLYFE
GERFNTYRLIRAVKNRFGSTNELGVFEMREIGLVELENPSKILIAEKPKDVAGSIIISTVEGTRPMLLELQALASPTSFG
IPKRTATGVDYNRVSLLMAVLEKRVGMQIQNQDIYINVVGGIKINEPSIDLGIVMAIASSFRNIPIDGNVAITGEVGLTG
EVRAVSFIEKRIAECKKLGFTKIVIPKSNYEAVKDVKGIDICPVDSVRQAINTVLGGNR

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=589083 KXZ80_RS00435 WP_021431480.1 50508..51887(+) (radA) [Paraclostridium bifermentans strain DSM 14991]
ATGGCTAAAATAAAAACTAAATATGTGTGTCAAGAATGTGGGTATGAGAATAGTAAATGGCTAGGAAAATGTCCAGAATG
TAGCAAATGGAATACATTTGTCGAAGAAATAGAAGAGAAAACTTCAAAATCAAATAAAGAAGTTTTTGTTATAGATAAAT
CATCATCTAGACCTTTAAATATAAACTCGATAGAAACTATAAAAGAGCAAAGATTTTCAACTTGCATAAACGAGTTAGAT
AGAGTTTTAGGTGGAGGTGTAGTTAAAGGATCATTAGTTTTAGTCGGAGGGGATCCAGGTATTGGGAAGTCAACGTTACT
TATACAAGTTTCAAGTAATGTTGCTAATAGTGGTAAAAAAGTTTTATACATATCAGGAGAAGAATCTGCATCTCAAATAA
AAATGAGGGCTCAAAGATTAGGAATAAAGTCCGATAATTTATATATATTTGCTGAAAATAACTTGAGCATAATAGAAGCT
CACTTAGAAAGTGTAAATCCAGACTTAATTATATTAGACTCTATACAAACTGTTTTTAGTCCTGAAATTACATCTGCTCC
AGGTACTGTAAGCCAGATAAAGGAAGGTACTTCGAGATTTATGAAAATTTCTAAAAAAATGGGTATATCAACCTTTGTAG
TTGGACATGTAACTAAAGAAGGTGCACTAGCAGGACCGAAGGTATTAGAACATATGGTAGACACTGTATTATATTTTGAA
GGAGAACGATTCAATACGTATAGATTGATAAGAGCTGTAAAAAATAGATTTGGGTCTACAAATGAATTAGGTGTATTTGA
GATGCGAGAAATAGGATTAGTTGAGTTAGAAAATCCATCTAAAATACTTATAGCCGAAAAACCAAAAGATGTAGCAGGGT
CTATAATTATATCTACAGTAGAAGGTACTAGACCAATGCTTTTAGAATTGCAAGCACTAGCATCACCTACTAGTTTTGGT
ATTCCTAAAAGAACGGCTACAGGAGTTGATTATAATAGAGTATCTTTACTTATGGCTGTTCTTGAAAAACGTGTTGGGAT
GCAAATACAAAACCAGGATATATACATAAATGTTGTTGGAGGAATAAAAATTAATGAGCCGTCTATAGATCTAGGCATAG
TTATGGCTATAGCTTCTAGTTTTAGAAACATACCTATAGATGGGAATGTAGCAATTACAGGAGAAGTTGGATTAACAGGA
GAAGTAAGAGCGGTGAGCTTTATAGAAAAGAGAATCGCAGAATGTAAGAAGCTAGGTTTCACTAAAATAGTTATTCCAAA
GAGCAATTATGAGGCTGTTAAAGATGTAAAAGGGATAGATATATGTCCAGTGGATAGTGTAAGACAAGCTATAAATACGG
TATTAGGAGGAAATAGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-221)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(349-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T4VZN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

57.424

99.782

0.573
  radA Streptococcus pneumoniae Rx1

52.193

99.346

0.519
  radA Streptococcus pneumoniae D39

52.193

99.346

0.519
  radA Streptococcus pneumoniae R6

52.193

99.346

0.519
  radA Streptococcus pneumoniae TIGR4

52.193

99.346

0.519
  radA Streptococcus mitis NCTC 12261

51.974

99.346

0.516
  radA Streptococcus mitis SK321

51.974

99.346

0.516