Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KX728_RS00130 Genome accession   NZ_CP079724
Coordinates   23151..24512 (+) Length   453 a.a.
NCBI ID   WP_084917279.1    Uniprot ID   -
Organism   Streptococcus oralis strain 34     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 18151..29512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KX728_RS00100 (KX728_00100) comW 19532..19744 (+) 213 WP_219108644.1 sigma(X)-activator ComW -
  KX728_RS00105 (KX728_00105) - 20010..21296 (+) 1287 WP_215805100.1 adenylosuccinate synthase -
  KX728_RS00110 (KX728_00110) tadA 21497..21964 (+) 468 WP_001110101.1 tRNA adenosine(34) deaminase TadA -
  KX728_RS00120 (KX728_00120) - 22153..22596 (+) 444 WP_000701998.1 dUTP diphosphatase -
  KX728_RS00125 (KX728_00125) - 22598..23137 (+) 540 WP_215805101.1 histidine phosphatase family protein -
  KX728_RS00130 (KX728_00130) radA 23151..24512 (+) 1362 WP_084917279.1 DNA repair protein RadA Machinery gene
  KX728_RS00135 (KX728_00135) - 24585..25079 (+) 495 WP_002876632.1 carbonic anhydrase -
  KX728_RS00140 (KX728_00140) - 25293..26261 (+) 969 WP_000010174.1 ribose-phosphate diphosphokinase -
  KX728_RS00145 (KX728_00145) - 26381..26818 (+) 438 WP_049490128.1 CoA-binding protein -
  KX728_RS00150 (KX728_00150) - 26847..27857 (-) 1011 WP_000009151.1 YeiH family protein -
  KX728_RS00155 (KX728_00155) - 28009..29178 (+) 1170 WP_084917280.1 pyridoxal phosphate-dependent aminotransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49451.92 Da        Isoelectric Point: 6.1894

>NTDB_id=588944 KX728_RS00130 WP_084917279.1 23151..24512(+) (radA) [Streptococcus oralis strain 34]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQMSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRSEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIEVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=588944 KX728_RS00130 WP_084917279.1 23151..24512(+) (radA) [Streptococcus oralis strain 34]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCTAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCTTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTTAACCGTGTG
CTTGGTGGAGGTGTGGTACCGGGGAGTCTCGTTCTTATCGGTGGGGATCCAGGGATTGGGAAATCAACCCTTCTCTTACA
AGTATCGACTCAGATGTCCCAAGTAGGGACTGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTCCGTG
CAGAGCGCTTGGGGGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGATCTGAGGTGGAG
CGCATCCAACCAGATTTTCTCATCATCGACTCTATCCAGACGATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
GTCTCAGGTGCGTGAGGTAACGGCTGAACTCATGCAGTTGGCGAAGACTAATAACATTGCTATCTTTATCGTAGGACATG
TGACTAAGGAAGGAACCTTGGCAGGTCCGCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGC
CACCATACCTTCCGTATTTTGAGGGCAGTCAAAAACCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGTGGATTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAGGAGCGTTTGGATGGGGCTACTGGCTCGTCAATCG
TTGTGACCATGGAAGGGACCCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCAACCATGTTTGGAAATGCTAAG
CGTACGACGACTGGACTTGATTTCAATCGTGCAAGTCTGATTATGGCTGTTTTGGAAAAACGAGCAGGGCTTCTTTTGCA
AAATCAGGATGCTTATCTCAAATCTGCTGGTGGCGTCAAATTGGATGAGCCTGCCATTGACTTAGCCGTTGCAGTGGCTA
TTGCCTCTAGTTACAAGGACAAGCCTACCAATCCTCAGGAATGTTTTGTGGGTGAACTGGGCTTGACCGGAGAAATTCGG
CGCGTGAATCGCATCGAACAACGCATAAATGAAGCGGCAAAACTGGGCTTTACCAAGATTTATGTACCCAAGAATTCCTT
GACAGGAATCACTCCACCCAAGGAAATTGAAGTCATTGGTGTGACAACGATTCAAGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.117

100

0.991
  radA Streptococcus pneumoniae Rx1

99.117

100

0.991
  radA Streptococcus pneumoniae D39

99.117

100

0.991
  radA Streptococcus pneumoniae R6

99.117

100

0.991
  radA Streptococcus pneumoniae TIGR4

99.117

100

0.991
  radA Bacillus subtilis subsp. subtilis str. 168

63.355

100

0.634