Detailed information NTG0058893

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: SPISAL_RS01080
• Genome accession: NC_021291
• Coordinates: 222275..223249 (-)
• Length: 324 a.a.
• NCBI ID: WP_016352623.1
• Uniprot ID: R4VDC4
• Organism: Spiribacter salinus M19-40
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 217275..228249

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPISAL_RS01050 (SPISAL_01055)	dut	217339..217794 (-)	456	WP_016352617.1	dUTP diphosphatase	-
SPISAL_RS01055 (SPISAL_01060)	coaBC	217781..218989 (-)	1209	WP_016352618.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
SPISAL_RS01060 (SPISAL_01065)	radC	219072..219746 (+)	675	WP_016352619.1	DNA repair protein RadC	-
SPISAL_RS01065 (SPISAL_01070)	rpoH	219718..220629 (-)	912	WP_144060419.1	RNA polymerase sigma factor RpoH	-
SPISAL_RS01070 (SPISAL_01075)	ftsX	220699..221631 (-)	933	WP_016352621.1	permease-like cell division protein FtsX	-
SPISAL_RS01075 (SPISAL_01080)	ftsE	221628..222278 (-)	651	WP_016352622.1	cell division ATP-binding protein FtsE	-
SPISAL_RS01080 (SPISAL_01085)	pilA	222275..223249 (-)	975	WP_016352623.1	signal recognition particle-docking protein FtsY	Machinery gene
SPISAL_RS01085 (SPISAL_01090)	-	223320..224657 (+)	1338	WP_016352624.1	pitrilysin family protein	-
SPISAL_RS01090 (SPISAL_01095)	-	224660..225979 (+)	1320	WP_016352625.1	pitrilysin family protein	-
SPISAL_RS01095 (SPISAL_01100)	rsmD	225984..226538 (+)	555	WP_016352626.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
SPISAL_RS01100 (SPISAL_01105)	coaD	226617..227129 (+)	513	WP_016352627.1	pantetheine-phosphate adenylyltransferase	-
SPISAL_RS01105 (SPISAL_01110)	-	227339..227620 (+)	282	WP_016352628.1	RNA-binding protein	-

Sequence

Protein

• Length: 324 a.a.
• Molecular weight: 34633.39 Da
• Isoelectric Point: 4.6133

>NTDB_id=58893 SPISAL_RS01080 WP_016352623.1 222275..223249(-) (pilA) [Spiribacter salinus M19-40]
MLPFRRKRETSEAPPAGLLQKLRSGLKRTGSGLGTLFLGRKQIDDALLEELETMLLMADVGVEATTQIIEDLTARLKRNE
LTDLDALQTALREDMLAILQPAESPLTIVEQDAPVVILVVGVNGAGKTTTIGKLAARLRSEGQRVILAAGDTFRAAAVEQ
LQTWGERTETPVISQPTGADSASVVYDAHQAATARGADVLIADTAGRLHTQGNLMEELRKIHRVLGKQDDTSPHEVLLVL
DAGNGQNALAQAEQFQQAVNVTGIALTKLDGTAKGGVIFAMAQRFGIPVRFIGIGEGVEDLRPFDAEAFVDALLSQAQTD
ADET

Nucleotide

• Length: 975 bp

>NTDB_id=58893 SPISAL_RS01080 WP_016352623.1 222275..223249(-) (pilA) [Spiribacter salinus M19-40]
ATGCTGCCATTTCGCCGTAAACGTGAAACCAGCGAAGCACCCCCTGCCGGCCTGCTGCAAAAGCTTCGCTCGGGGCTGAA
ACGCACCGGCAGCGGGCTTGGCACCCTCTTTCTGGGGCGCAAGCAGATTGATGACGCGCTCCTCGAAGAGCTGGAGACGA
TGCTGCTCATGGCCGACGTCGGGGTTGAGGCGACGACCCAGATCATTGAGGACCTCACAGCCCGGCTCAAGCGCAACGAA
CTCACGGATCTCGACGCCCTGCAAACGGCCTTGCGCGAGGACATGCTGGCCATCCTTCAGCCCGCTGAATCACCGCTCAC
CATCGTCGAGCAGGACGCGCCCGTGGTCATTCTGGTGGTGGGCGTCAACGGCGCCGGCAAAACCACCACCATCGGCAAGC
TGGCGGCGCGGCTGCGCAGCGAGGGCCAACGGGTCATTCTGGCCGCTGGCGACACCTTCCGGGCGGCCGCTGTCGAGCAA
TTGCAAACCTGGGGCGAGCGCACCGAAACCCCCGTAATTAGCCAGCCAACCGGGGCAGACAGCGCGTCCGTTGTGTACGA
CGCCCACCAGGCAGCCACAGCGCGGGGCGCCGATGTGCTCATCGCCGACACGGCGGGGCGTCTGCACACCCAGGGCAACC
TGATGGAGGAGCTGCGCAAAATCCACCGGGTGCTCGGCAAACAGGATGACACCTCACCGCACGAGGTCCTGCTGGTGCTC
GATGCCGGTAACGGACAAAACGCACTCGCACAGGCCGAACAGTTTCAGCAGGCCGTGAACGTCACCGGCATCGCCCTCAC
CAAGCTCGACGGGACGGCCAAGGGCGGCGTGATCTTTGCGATGGCTCAGCGCTTCGGTATCCCCGTGCGCTTTATTGGCA
TCGGTGAGGGCGTGGAAGATTTGCGCCCGTTCGATGCCGAGGCCTTCGTGGACGCGCTGCTCAGCCAGGCGCAAACCGAC
GCCGATGAGACATGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (114-314)

• SRP54-type protein, helical bundle domain (SRP54_N) (30-94)

3D structure

• AlphaFold DB: R4VDC4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.456	95.37	0.491