Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BRPE64_RS01125 Genome accession   NC_021287
Coordinates   233678..234985 (-) Length   435 a.a.
NCBI ID   WP_016344156.1    Uniprot ID   R4WF23
Organism   Caballeronia insecticola     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228678..239985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRPE64_RS01105 (BRPE64_ACDS02310) ybiB 230363..231334 (-) 972 WP_016344152.1 DNA-binding protein YbiB -
  BRPE64_RS01110 (BRPE64_ACDS02320) - 231639..232337 (+) 699 WP_016344153.1 fumarylacetoacetate hydrolase family protein -
  BRPE64_RS01115 (BRPE64_ACDS02330) maiA 232348..232992 (+) 645 WP_044041871.1 maleylacetoacetate isomerase -
  BRPE64_RS01120 (BRPE64_ACDS02340) - 232989..233621 (+) 633 WP_016344155.1 LysE/ArgO family amino acid transporter -
  BRPE64_RS01125 (BRPE64_ACDS02350) pilA 233678..234985 (-) 1308 WP_016344156.1 signal recognition particle-docking protein FtsY Machinery gene
  BRPE64_RS01130 (BRPE64_ACDS02360) rsmD 235199..235825 (+) 627 WP_044041054.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BRPE64_RS01135 (BRPE64_ACDS02370) coaD 235940..236443 (+) 504 WP_016344158.1 pantetheine-phosphate adenylyltransferase -
  BRPE64_RS01140 (BRPE64_ACDS02380) - 236523..236780 (+) 258 WP_044041056.1 YfhL family 4Fe-4S dicluster ferredoxin -
  BRPE64_RS01145 (BRPE64_ACDS02390) hisC 236807..237874 (-) 1068 WP_016344160.1 histidinol-phosphate transaminase -
  BRPE64_RS01150 (BRPE64_ACDS02400) pth 237903..238502 (-) 600 WP_016344161.1 aminoacyl-tRNA hydrolase -
  BRPE64_RS01155 (BRPE64_ACDS02410) - 238634..239254 (-) 621 WP_044041058.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 435 a.a.        Molecular weight: 46532.92 Da        Isoelectric Point: 4.7024

>NTDB_id=58851 BRPE64_RS01125 WP_016344156.1 233678..234985(-) (pilA) [Caballeronia insecticola]
MFSFFKRFKKPADGPLDTPQDESAEALDDEELSDDELERQALAAEAPPEAPPVAAREKAESAAPVPAQPVPAPAPAPSAP
SAPAPAKPAAPPSQKAESAEESKEYWQGRTQSQWSRAPATDLASEEFVPPPEPDPAAKKSWLSRLRHGLSKTSSNLTGIF
VGAKIDEDLYEELETALLMSDAGVDATEFLLESLREKVRSERLTDASQVKAALRDLLIDLLRPLEKSLMLGRAQPLVMMI
AGVNGAGKTTSIGKLAKHLQHFNQSVLLAAGDTFRAAAREQLTVWGERNNVTVIAQESGDAAAVIFDAVGAARARKIDVM
MADTAGRLPTQLHLMEELRKVRRVIAKAMPDAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGIIAA
IARQRPIPVYFIGVGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1308 bp        

>NTDB_id=58851 BRPE64_RS01125 WP_016344156.1 233678..234985(-) (pilA) [Caballeronia insecticola]
ATGTTCAGCTTCTTCAAACGATTCAAAAAGCCGGCTGACGGGCCGCTCGACACGCCTCAGGACGAATCCGCCGAGGCGCT
TGACGACGAAGAGCTTTCCGACGACGAGCTGGAACGGCAGGCGCTCGCAGCCGAGGCGCCGCCGGAAGCGCCGCCTGTAG
CCGCGCGCGAAAAGGCCGAGTCTGCTGCACCGGTGCCTGCGCAGCCTGTGCCCGCACCGGCGCCCGCGCCTTCTGCGCCT
TCTGCGCCCGCGCCCGCCAAACCCGCCGCCCCGCCGTCGCAAAAGGCAGAAAGCGCAGAGGAATCGAAGGAATACTGGCA
AGGCCGGACGCAGTCGCAATGGTCGCGCGCGCCCGCAACCGATCTCGCGAGCGAAGAGTTCGTGCCGCCGCCCGAGCCGG
ATCCCGCCGCGAAGAAATCGTGGCTTTCCCGTTTGCGCCACGGTCTCTCGAAGACGAGTTCGAACCTGACCGGCATCTTC
GTCGGCGCGAAAATCGACGAGGATTTGTACGAAGAGCTCGAAACCGCGCTGCTGATGTCCGACGCCGGCGTCGACGCGAC
CGAATTCCTGCTCGAATCGCTACGGGAAAAAGTGCGCTCGGAGCGTCTGACCGACGCGTCGCAGGTGAAGGCCGCGCTGC
GCGATCTGCTGATCGACCTGCTGCGTCCGCTCGAAAAGTCGCTGATGCTCGGCCGCGCCCAACCGCTCGTGATGATGATC
GCGGGCGTGAACGGCGCCGGCAAGACGACCAGCATCGGCAAGCTGGCGAAGCATCTGCAGCACTTCAATCAGTCGGTGCT
GCTCGCCGCCGGCGACACTTTCCGCGCCGCCGCGCGCGAGCAGTTGACCGTCTGGGGCGAGCGCAACAACGTCACGGTGA
TCGCGCAGGAAAGCGGCGATGCGGCCGCCGTCATCTTCGACGCGGTCGGTGCGGCACGCGCGCGCAAGATCGACGTGATG
ATGGCCGACACCGCCGGCCGCCTGCCGACGCAACTGCATCTGATGGAAGAGCTGCGCAAGGTGCGCCGCGTCATCGCCAA
AGCCATGCCCGACGCGCCTCACGAAGTGCTGCTCGTGATCGATGCGAACACGGGTCAGAACGCGCTCGCGCAAGTGAAGG
CGTTCGACGACGCGCTGGGCCTGACCGGTCTCATCGTCACGAAGCTGGATGGCACGGCAAAAGGCGGCATCATCGCGGCG
ATCGCGCGGCAGCGCCCGATTCCGGTGTATTTCATCGGCGTGGGCGAGAAAGTCGAAGACTTGCAGCCGTTCAGCGCGGA
AGAATTCGCGGACGCGCTGCTTGGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(236-435)

SRP54-type protein, helical bundle domain (SRP54_N)

(155-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R4WF23

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.797

100

0.513

Multiple sequence alignment