Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KVY05_RS13705 Genome accession   NZ_CP079099
Coordinates   2746696..2747310 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBY-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2741696..2752310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KVY05_RS13675 (KVY05_13675) rpsT 2741999..2742265 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  KVY05_RS13680 (KVY05_13680) holA 2742282..2743323 (-) 1042 Protein_2679 DNA polymerase III subunit delta -
  KVY05_RS13685 (KVY05_13685) - 2743363..2743514 (-) 152 Protein_2680 hypothetical protein -
  KVY05_RS13690 (KVY05_13690) - 2743555..2743689 (+) 135 WP_003152870.1 YqzM family protein -
  KVY05_RS13695 (KVY05_13695) comEC 2743708..2746059 (-) 2352 WP_015240244.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KVY05_RS13700 (KVY05_13700) - 2746060..2746629 (-) 570 WP_003152868.1 ComE operon protein 2 -
  KVY05_RS13705 (KVY05_13705) comEA 2746696..2747310 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  KVY05_RS13710 (KVY05_13710) comER 2747369..2748190 (+) 822 WP_015240246.1 late competence protein ComER -
  KVY05_RS13715 (KVY05_13715) - 2748259..2748996 (-) 738 WP_015240247.1 class I SAM-dependent methyltransferase -
  KVY05_RS13720 (KVY05_13720) rsfS 2748993..2749349 (-) 357 WP_007408260.1 ribosome silencing factor -
  KVY05_RS13725 (KVY05_13725) yqeK 2749367..2749927 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KVY05_RS13730 (KVY05_13730) - 2749917..2750486 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  KVY05_RS13735 (KVY05_13735) yhbY 2750497..2750787 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  KVY05_RS13740 (KVY05_13740) aroE 2750781..2751623 (-) 843 WP_015240248.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=587755 KVY05_RS13705 WP_015240245.1 2746696..2747310(-) (comEA) [Bacillus velezensis strain LBY-1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=587755 KVY05_RS13705 WP_015240245.1 2746696..2747310(-) (comEA) [Bacillus velezensis strain LBY-1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363