Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KV103_RS12050 Genome accession   NZ_CP078149
Coordinates   2518605..2519219 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain BS-G1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513605..2524219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KV103_RS12020 rpsT 2513908..2514174 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  KV103_RS12025 holA 2514191..2515232 (-) 1042 Protein_2343 DNA polymerase III subunit delta -
  KV103_RS12030 - 2515272..2515423 (-) 152 Protein_2344 hypothetical protein -
  KV103_RS12035 - 2515464..2515598 (+) 135 WP_003152870.1 YqzM family protein -
  KV103_RS12040 comEC 2515617..2517968 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KV103_RS12045 - 2517969..2518538 (-) 570 WP_003152868.1 ComE operon protein 2 -
  KV103_RS12050 comEA 2518605..2519219 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  KV103_RS12055 comER 2519278..2520099 (+) 822 WP_012118027.1 late competence protein ComER -
  KV103_RS12060 - 2520168..2520905 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  KV103_RS12065 rsfS 2520902..2521258 (-) 357 WP_007408260.1 ribosome silencing factor -
  KV103_RS12070 yqeK 2521262..2521837 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KV103_RS12075 - 2521827..2522396 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  KV103_RS12080 yhbY 2522407..2522697 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  KV103_RS12085 aroE 2522691..2523533 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=587370 KV103_RS12050 WP_032873955.1 2518605..2519219(-) (comEA) [Bacillus velezensis strain BS-G1]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=587370 KV103_RS12050 WP_032873955.1 2518605..2519219(-) (comEA) [Bacillus velezensis strain BS-G1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363