Detailed information NTG0587266

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HQ966_RS12225
• Genome accession: NZ_CP078139
• Coordinates: 2670039..2671208 (+)
• Length: 389 a.a.
• NCBI ID: WP_002553335.1
• Uniprot ID: A0A554H2G7
• Organism: Pseudomonas savastanoi pv. savastanoi strain PVFi1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2665039..2676208

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HQ966_RS12210 (HQ966_12095)	-	2665476..2667803 (+)	2328	WP_002553332.1	ATP-dependent DNA helicase	-
HQ966_RS12215 (HQ966_12100)	pgm	2667919..2669565 (+)	1647	WP_031594726.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
HQ966_RS12220 (HQ966_12105)	-	2669640..2669915 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
HQ966_RS12225 (HQ966_12110)	pilU	2670039..2671208 (+)	1170	WP_002553335.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HQ966_RS12230 (HQ966_12115)	-	2671259..2672836 (-)	1578	WP_031594725.1	ABC transporter ATP-binding protein	-
HQ966_RS12235 (HQ966_12120)	-	2672838..2673860 (-)	1023	WP_005732025.1	ABC transporter permease	-
HQ966_RS12240 (HQ966_12125)	-	2673860..2674924 (-)	1065	WP_002553338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42548.76 Da
• Isoelectric Point: 6.0641

>NTDB_id=587266 HQ966_RS12225 WP_002553335.1 2670039..2671208(+) (pilU) [Pseudomonas savastanoi pv. savastanoi strain PVFi1]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTAPPPAPIGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=587266 HQ966_RS12225 WP_002553335.1 2670039..2671208(+) (pilU) [Pseudomonas savastanoi pv. savastanoi strain PVFi1]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAACCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCTGTACTGCTCGATGTGATCATGGAAAAGCACGGCCTGGTGTTGTTCGTCGGTGCCACCGGCT
CGGGCAAATCGACCTCGTTGGCAGCGCTGATCGATTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGGCGCCGCCCCCGGCCCCGATCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

3D structure

• AlphaFold DB: A0A554H2G7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.948	98.458	0.393
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362