Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KU891_RS20355 Genome accession   NZ_CP078081
Coordinates   3971257..3972300 (-) Length   347 a.a.
NCBI ID   WP_001013210.1    Uniprot ID   A0A5C5AE28
Organism   Bacillus tropicus strain EMB20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3966257..3977300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU891_RS20310 (KU891_20245) - 3966686..3966886 (-) 201 WP_000106081.1 YqzE family protein -
  KU891_RS20315 (KU891_20250) aroK 3966925..3967422 (-) 498 WP_000836642.1 shikimate kinase AroK -
  KU891_RS20320 (KU891_20255) - 3967541..3968191 (-) 651 WP_071678892.1 2OG-Fe(II) oxygenase -
  KU891_RS20325 (KU891_20260) comGG 3968367..3968738 (-) 372 WP_001231196.1 competence type IV pilus minor pilin ComGG -
  KU891_RS20330 (KU891_20265) comGF 3968735..3969205 (-) 471 WP_000923091.1 competence type IV pilus minor pilin ComGF -
  KU891_RS20335 (KU891_20270) comGE 3969175..3969477 (-) 303 WP_248139761.1 competence type IV pilus minor pilin ComGE -
  KU891_RS20340 (KU891_20275) comGD 3969470..3969925 (-) 456 WP_000810384.1 comG operon protein ComGD -
  KU891_RS20345 (KU891_20280) comGC 3969922..3970221 (-) 300 WP_001178695.1 comG operon protein ComGC -
  KU891_RS20350 (KU891_20285) comGB 3970233..3971264 (-) 1032 WP_071678893.1 competence type IV pilus assembly protein ComGB -
  KU891_RS20355 (KU891_20290) comGA 3971257..3972300 (-) 1044 WP_001013210.1 competence protein ComGA Machinery gene
  KU891_RS20360 (KU891_20295) - 3972505..3973200 (+) 696 WP_029439920.1 helix-turn-helix domain-containing protein -
  KU891_RS20365 (KU891_20300) - 3973326..3973568 (+) 243 WP_000440713.1 DUF2626 family protein -
  KU891_RS20370 (KU891_20305) - 3973676..3975070 (+) 1395 WP_001094339.1 L-cystine transporter -
  KU891_RS20375 (KU891_20310) - 3975286..3975501 (-) 216 WP_001008328.1 DUF3912 family protein -
  KU891_RS20380 (KU891_20315) - 3975754..3976065 (+) 312 WP_001093243.1 hypothetical protein -
  KU891_RS20385 (KU891_20320) - 3976102..3976590 (-) 489 WP_000764271.1 hypothetical protein -
  KU891_RS20390 (KU891_20325) - 3976670..3977044 (-) 375 WP_071678894.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39304.72 Da        Isoelectric Point: 8.7292

>NTDB_id=586611 KU891_RS20355 WP_001013210.1 3971257..3972300(-) (comGA) [Bacillus tropicus strain EMB20]
MNGIESFANTILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRSDDLLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGTILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCQKKCSTVCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=586611 KU891_RS20355 WP_001013210.1 3971257..3972300(-) (comGA) [Bacillus tropicus strain EMB20]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGACAGAAGGATGTAGTGGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTACGTCTTTCCACGCTTCCAACTGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGGTTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGCCGCATCGTTACACTGGAGGATCCAGTTGAAAAAAGAAGTGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACACTGCAT
ACAAATGATGCGAGAGGGACGATACTCAGGTTCATGGATTTTGGCATAACGAGGCAAGAGATTGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTTGAATTAAAGTGTCCGTTTTGCCAAAAAAAGTGCTCAACTGTATGCAAATCAATGAGGC
AAGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAACACGAAACATTAGAATCTTCGATACGAAAAGGATACGCTTTAGGATTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5AE28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576
  pilB Vibrio campbellii strain DS40M4

36.261

100

0.369