Detailed information NTG0586592

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: KU891_RS05750
• Genome accession: NZ_CP078081
• Coordinates: 1131318..1135043 (+)
• Length: 1241 a.a.
• NCBI ID: WP_139888658.1
• Uniprot ID: -
• Organism: Bacillus tropicus strain EMB20
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1126318..1140043

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KU891_RS05735 (KU891_05730)	-	1126476..1127069 (+)	594	WP_139888660.1	TVP38/TMEM64 family protein	-
KU891_RS05740 (KU891_05735)	lepB	1127126..1127689 (+)	564	WP_000751911.1	signal peptidase I	-
KU891_RS05745 (KU891_05740)	addB	1127806..1131321 (+)	3516	WP_139888659.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
KU891_RS05750 (KU891_05745)	addA	1131318..1135043 (+)	3726	WP_139888658.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
KU891_RS05755 (KU891_05750)	-	1135056..1135343 (+)	288	WP_061403212.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
KU891_RS05760 (KU891_05755)	gerPF	1135424..1135639 (-)	216	WP_001141566.1	spore germination protein GerPF	-
KU891_RS05765 (KU891_05760)	-	1135682..1136068 (-)	387	WP_000902340.1	spore germination protein GerPE	-
KU891_RS05770 (KU891_05765)	gerPD	1136084..1136278 (-)	195	WP_001102341.1	spore germination protein GerPD	-
KU891_RS05775 (KU891_05770)	gerPC	1136285..1136899 (-)	615	WP_001070744.1	spore germination protein GerPC	-
KU891_RS05780 (KU891_05775)	gerPB	1136968..1137174 (-)	207	WP_001012508.1	spore germination protein GerPB	-
KU891_RS05785 (KU891_05780)	gerPA	1137189..1137410 (-)	222	WP_001111187.1	spore germination protein GerPA	-
KU891_RS05790 (KU891_05785)	-	1137507..1137686 (-)	180	WP_000462840.1	aspartyl-phosphate phosphatase Spo0E family protein	-
KU891_RS05795 (KU891_05790)	-	1137934..1138833 (+)	900	WP_001241720.1	fumarylacetoacetate hydrolase family protein	-
KU891_RS05800 (KU891_05795)	-	1138873..1139157 (-)	285	WP_000925336.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142840.31 Da
• Isoelectric Point: 4.8687

>NTDB_id=586592 KU891_RS05750 WP_139888658.1 1131318..1135043(+) (addA) [Bacillus tropicus strain EMB20]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQTMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATAHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVILFFES
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=586592 KU891_RS05750 WP_139888658.1 1131318..1135043(+) (addA) [Bacillus tropicus strain EMB20]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAGGCGTTAGAAAAAGTATTAATTGATGAGCCGGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCCAATCAAACAGAAAATGAACTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCCTACTTATTAGAAGATGTGAAATTTCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGTTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGCTATTTAGCCGTAAGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAGCTCGTAAAAGTATTTACAGAACGTTTCCAAACGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGGAAGTATAAACGTTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATTGACCTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACGGAAGAAGAGGTAATAGACGGTGAAGAAGGCGCGGAAGTAGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCTGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGGCCTGTACAATA
CCGCGACTTCGTTATTTTGCTCCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCCGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGCGCACCGCTTGAAGAAGAAAAAGAAT
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTACGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCAAGAGCTTTAGGTGAACAAGAAGATGTTGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGTCGCTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAATTTATTGATCCACGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATATGTTGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGCTATTACCAGATCATATACGTGCCGGAGCGTCTTGTTACTTAGACTGGATTGCCCCTTCCTTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTTGCGCCAGAACCAGTTCAAGAAGAGAAGCAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCCGTTCCCCTGCAAAGTGAACGGAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACAGCTCATCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGAACACGTCCTCGTTTTATGGAGAAAAAAGGTTTAACGTACGCAGAGCGTGG
AACCGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGATTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTTGAACAAGCAGAAGAAATAGCGATTGAAAAAGTAATTTTATTCTTTGAAAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGTGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.747	100	0.537