Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KU891_RS00530 Genome accession   NZ_CP078081
Coordinates   97547..98923 (+) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus tropicus strain EMB20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92547..103923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU891_RS00510 (KU891_00510) ctsR 92740..93201 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  KU891_RS00515 (KU891_00515) - 93375..93923 (+) 549 WP_061403146.1 UvrB/UvrC motif-containing protein -
  KU891_RS00520 (KU891_00520) - 93928..94992 (+) 1065 WP_000050825.1 protein arginine kinase -
  KU891_RS00525 (KU891_00525) clpC 95015..97450 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  KU891_RS00530 (KU891_00530) radA 97547..98923 (+) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  KU891_RS00535 (KU891_00535) disA 98927..100000 (+) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  KU891_RS00540 (KU891_00540) - 100163..101272 (+) 1110 WP_061403145.1 PIN/TRAM domain-containing protein -
  KU891_RS00545 (KU891_00545) ispD 101289..101969 (+) 681 WP_000288291.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KU891_RS00550 (KU891_00550) ispF 102085..102561 (+) 477 WP_000488394.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=586586 KU891_RS00530 WP_001085212.1 97547..98923(+) (radA) [Bacillus tropicus strain EMB20]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=586586 KU891_RS00530 WP_001085212.1 97547..98923(+) (radA) [Bacillus tropicus strain EMB20]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCGAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGATTCTATTCAAACAATACATTTACCTGAAGTAACGTCAGC
GCCAGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTCGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCGATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCGGAAGTATTAAACCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGGGTGGCGG
GGTCAACAGTGGTTGCTTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGATGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCGACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGGTTTCAACGCGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACCATTCCAGATGGGATCGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629