Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KUD94_RS11405 Genome accession   NZ_CP078069
Coordinates   2380970..2382502 (+) Length   510 a.a.
NCBI ID   WP_218237325.1    Uniprot ID   -
Organism   Comamonas sp. NLF-1-9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2375970..2387502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUD94_RS11380 (KUD94_11380) argS 2376357..2378036 (+) 1680 WP_218237320.1 arginine--tRNA ligase -
  KUD94_RS11385 (KUD94_11385) - 2378042..2378695 (+) 654 WP_218237321.1 SPOR domain-containing protein -
  KUD94_RS11390 (KUD94_11390) - 2378766..2379413 (+) 648 WP_218237322.1 thiol:disulfide interchange protein DsbA/DsbL -
  KUD94_RS11395 (KUD94_11395) lptA 2379542..2380171 (+) 630 WP_218237323.1 lipopolysaccharide transport periplasmic protein LptA -
  KUD94_RS11400 (KUD94_11400) lptB 2380177..2380965 (+) 789 WP_218237324.1 LPS export ABC transporter ATP-binding protein -
  KUD94_RS11405 (KUD94_11405) rpoN/rpoN1 2380970..2382502 (+) 1533 WP_218237325.1 RNA polymerase factor sigma-54 Machinery gene
  KUD94_RS11410 (KUD94_11410) - 2382526..2383791 (+) 1266 WP_218237326.1 class I SAM-dependent RNA methyltransferase -
  KUD94_RS11415 (KUD94_11415) - 2383915..2384661 (+) 747 WP_218237327.1 M15 family metallopeptidase -
  KUD94_RS11420 (KUD94_11420) - 2384686..2385081 (-) 396 WP_218237328.1 cupin domain-containing protein -
  KUD94_RS11425 (KUD94_11425) - 2385155..2386945 (-) 1791 WP_218237329.1 alpha/beta fold hydrolase -
  KUD94_RS11430 (KUD94_11430) - 2386990..2387433 (+) 444 WP_218237330.1 putative toxin-antitoxin system toxin component, PIN family -

Sequence

Protein


Download         Length: 510 a.a.        Molecular weight: 56475.73 Da        Isoelectric Point: 5.0214

>NTDB_id=586548 KUD94_RS11405 WP_218237325.1 2380970..2382502(+) (rpoN/rpoN1) [Comamonas sp. NLF-1-9]
MKVGLSLGVSQHLALTPQLQQSIRLLQLSTLELAQEVEAMLIDNPFLERAGEEPPAAGAPAPERRPERSEIAPISEAFDA
DPESAGSQNSAETRSDWEGDRELDPSGADSPWSSEAPGRAARREDDESEASEHESAHETLAEHLHRQALLLRLTRAERAA
LSWLIDSLNDDGYLEDAIESLVETLVGPENTEYLLEMSEHFALALKLLQSLEPAGVGARSLAECLQLQLAALRDAGACPR
AVLDAAMCICEQPLEWLARRDVRRLSQACGASEEDTRAAMALIARLEPRPGRRFAQVQHNLVTPDVLVRRRLEGGQVRLD
VQLNPEVVPQLRVHDAYAQALRQHRKDEGHAALQSRLQEARWFIRNVEQRFDTILRTARAIVDKQQPFFLHGELAMRPLV
MREIAEELGLHESTISRVTSAKYMATPQGTYELKYFFGSGLGTDTGGSTSSTAVRALLRQFIAAEDPARPLSDGQLADML
KEQGIACARRTVAKYREQLKIAPASLRRQV

Nucleotide


Download         Length: 1533 bp        

>NTDB_id=586548 KUD94_RS11405 WP_218237325.1 2380970..2382502(+) (rpoN/rpoN1) [Comamonas sp. NLF-1-9]
ATGAAGGTCGGCCTGTCGCTGGGCGTCTCGCAACACCTTGCGCTGACGCCGCAACTGCAGCAGTCCATTCGCCTGCTGCA
ACTGTCCACGCTGGAGCTGGCGCAGGAGGTGGAGGCCATGCTCATCGACAACCCCTTCCTCGAGCGCGCGGGCGAGGAGC
CCCCCGCAGCCGGCGCGCCCGCGCCGGAGCGGCGCCCCGAGCGCAGCGAGATTGCTCCTATTTCAGAAGCATTTGACGCA
GACCCGGAAAGCGCTGGAAGCCAAAACAGTGCCGAAACCCGGAGCGACTGGGAAGGCGACCGCGAGCTCGACCCCAGCGG
CGCCGACAGCCCCTGGAGCAGCGAGGCCCCGGGCCGCGCCGCCCGGCGCGAGGACGATGAGAGCGAGGCGAGCGAGCACG
AGAGCGCGCACGAAACCCTGGCCGAGCACCTGCACCGCCAGGCGCTGCTGCTGCGCCTCACCCGCGCCGAACGCGCGGCG
CTGTCCTGGCTGATCGATTCGCTCAACGACGACGGCTACCTCGAAGACGCCATCGAATCGCTGGTCGAAACCCTGGTCGG
CCCCGAGAACACCGAATACCTGCTGGAGATGAGCGAGCACTTTGCGCTGGCGCTCAAGCTGCTGCAAAGCCTGGAGCCCG
CCGGTGTCGGCGCGCGCAGCCTGGCCGAATGCCTGCAACTGCAACTGGCGGCGCTGCGCGACGCGGGCGCCTGCCCGCGG
GCGGTGCTGGATGCGGCCATGTGCATCTGCGAGCAGCCGCTTGAGTGGCTCGCGCGGCGCGACGTGCGGCGCCTGTCGCA
GGCCTGCGGCGCGAGCGAGGAGGACACGCGCGCCGCCATGGCGCTGATCGCCCGGCTGGAACCGCGCCCGGGGCGGCGCT
TTGCCCAGGTGCAGCACAACCTCGTCACGCCCGACGTGCTCGTGCGCCGCCGCCTGGAGGGCGGGCAGGTGCGCCTGGAC
GTGCAGCTCAACCCCGAAGTGGTGCCGCAGCTGCGCGTGCACGACGCCTACGCCCAGGCGCTGCGCCAGCACCGCAAGGA
CGAAGGCCACGCCGCGCTGCAAAGCCGCCTGCAGGAGGCGCGCTGGTTCATCAGGAACGTCGAGCAGCGCTTTGACACCA
TCTTGCGCACCGCGCGCGCCATCGTGGACAAGCAGCAGCCCTTCTTCCTGCATGGCGAGCTCGCCATGCGTCCGCTGGTG
ATGCGCGAGATTGCCGAGGAGCTGGGCCTGCACGAATCGACCATCTCGCGCGTGACCAGCGCCAAATACATGGCCACGCC
CCAGGGCACCTACGAACTGAAGTACTTCTTCGGCTCGGGCCTGGGCACCGACACCGGCGGCAGCACCTCCAGCACCGCGG
TGCGCGCGCTGCTGCGCCAGTTCATCGCCGCCGAAGACCCGGCGCGGCCGCTGTCCGACGGGCAACTGGCCGACATGCTC
AAGGAGCAAGGCATCGCCTGCGCGCGGCGCACCGTGGCCAAATACCGCGAACAGCTCAAGATTGCGCCCGCCAGCCTGCG
CCGCCAGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(353-508)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-337)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.51

100

0.486