Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   EVX74_RS14305 Genome accession   NZ_CP078045
Coordinates   2954837..2957059 (+) Length   740 a.a.
NCBI ID   WP_004729357.1    Uniprot ID   A0AAJ4P473
Organism   Acinetobacter lwoffii strain AL_065     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2949837..2962059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVX74_RS14285 (EVX74_014195) lnt 2950621..2952180 (+) 1560 WP_004729360.1 apolipoprotein N-acyltransferase -
  EVX74_RS14290 (EVX74_014200) - 2952347..2952682 (-) 336 WP_004729359.1 hypothetical protein -
  EVX74_RS14295 (EVX74_014205) gspG 2952967..2953401 (-) 435 WP_004278567.1 type II secretion system major pseudopilin GspG -
  EVX74_RS14300 (EVX74_014210) gspF 2953481..2954686 (-) 1206 WP_004729358.1 type II secretion system inner membrane protein GspF -
  EVX74_RS14305 (EVX74_014215) priA 2954837..2957059 (+) 2223 WP_004729357.1 primosomal protein N' Machinery gene
  EVX74_RS14310 (EVX74_014220) - 2957135..2958988 (+) 1854 WP_004729354.1 cation:proton antiporter -
  EVX74_RS14315 (EVX74_014225) - 2959039..2959914 (+) 876 WP_004278571.1 Hsp33 family molecular chaperone HslO -
  EVX74_RS14320 (EVX74_014230) - 2960236..2960697 (+) 462 WP_004647343.1 DUF2147 domain-containing protein -
  EVX74_RS14325 (EVX74_014235) - 2961182..2961511 (+) 330 WP_004278573.1 chaperone modulator CbpM -
  EVX74_RS14330 (EVX74_014240) - 2961601..2961864 (+) 264 WP_004729352.1 YdcH family protein -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 84144.16 Da        Isoelectric Point: 8.5527

>NTDB_id=586522 EVX74_RS14305 WP_004729357.1 2954837..2957059(+) (priA) [Acinetobacter lwoffii strain AL_065]
MQNSHTDSSLLYRVRVAVPVHVFDCFDYTMSAEQFEQAQIGARVLVSFGRQNLVGVIIEKLPADTPLDPRFKLKAVTELL
DERAIIDAKVLSLLTWSAQYYQFPIGEVVHSALPTLLRQGKPYNLLARTWKLLDPDAEAKVRRSDKQQEAYRILKLHPVG
TTENVLNMAGIETATLKALEKKEICACVLEPQDFNPQPMQLAQMPLTANPEQKHAVEQVLKYRNQYQAFLLDGLTGSGKT
EVYLQIMEQVLKQGKQVLVLVPEIGLTPQTISRFQSRFHCHIALLHSGLNDSKRLQAWQSAETGKASIVLGTRSAIYTPM
PNLGLIILDEEHDLSFKQQEGFRYHARDVALYRGHLQQCPVILGSATPSIDSYALVQHGKMQALELNQRAGTALMPKIHI
LDLKVAQKKHGISLQLIQDIKKRLEKKEQVLIFLNRRGYAPVLLCGSCGWQAQCPHCDANFTVHRQPYQHLHCHHCGTIH
RMPEHCPQCQHQELVTLGMGTGKVEEHLNELFPDFEVIRVDRDSTSRVGSWQKIYDKIQKSEPAILLGTQMLAKGHHFPY
VTLVAILDIDSGLLSVDFRATERTAQLIVQVAGRAGRGEHKGDVYLQTLRPDHALLNTLVNENYRVFAQQTLKERQMAKM
PPYRYAILIRCESKDQSQNTEFLQKHAALLRQYPDLALDIWGPIPAPMERKAGRYQSHMVLLSADRPRLHYYVRSWWQNM
LQDKPSSMKLTLDIDPQELS

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=586522 EVX74_RS14305 WP_004729357.1 2954837..2957059(+) (priA) [Acinetobacter lwoffii strain AL_065]
ATGCAAAATTCTCACACCGATTCCAGTCTACTTTATCGCGTACGCGTTGCTGTACCTGTACATGTATTTGATTGTTTTGA
TTACACAATGAGTGCAGAACAATTTGAACAGGCGCAGATTGGCGCACGTGTACTGGTATCGTTTGGTCGGCAGAATCTGG
TCGGGGTGATTATCGAAAAACTGCCTGCAGACACTCCTCTCGACCCACGCTTTAAGCTTAAGGCAGTAACCGAACTGCTC
GATGAACGCGCCATTATAGATGCCAAAGTTTTAAGTTTGTTGACATGGTCAGCGCAGTATTACCAGTTTCCGATCGGTGA
AGTCGTCCACAGTGCCCTGCCGACTCTGTTGCGTCAGGGTAAGCCTTATAACCTGCTGGCCCGTACCTGGAAATTACTGG
ACCCTGATGCCGAGGCCAAAGTACGCCGTTCGGACAAGCAGCAGGAAGCTTACCGGATTTTAAAACTGCATCCGGTCGGG
ACCACTGAAAATGTGCTGAACATGGCCGGGATTGAAACCGCGACCTTAAAAGCGCTGGAGAAAAAAGAGATTTGCGCCTG
TGTGCTGGAGCCACAAGATTTCAATCCGCAGCCGATGCAGCTGGCACAAATGCCGCTTACTGCCAATCCTGAACAAAAAC
ATGCAGTCGAACAGGTATTAAAATATCGCAATCAATATCAAGCTTTTTTACTGGATGGTCTGACCGGCAGCGGTAAAACT
GAAGTGTATTTGCAGATCATGGAACAGGTACTGAAACAGGGCAAACAGGTGCTGGTGCTGGTGCCGGAAATCGGCCTGAC
GCCGCAAACCATCAGCCGTTTTCAGTCCCGTTTTCATTGCCATATTGCGTTGCTGCATTCCGGGCTGAATGATTCCAAAC
GGCTACAAGCCTGGCAGTCGGCAGAAACCGGCAAAGCCTCAATCGTACTCGGTACACGTTCTGCCATTTATACCCCGATG
CCGAATCTGGGCCTGATCATTCTGGATGAAGAACATGACCTGTCCTTTAAACAGCAGGAAGGCTTCCGCTATCATGCCCG
CGATGTCGCCCTGTACCGCGGCCATTTACAGCAATGTCCGGTAATCTTAGGGTCAGCCACACCAAGCATTGACAGTTATG
CGCTGGTGCAACATGGCAAGATGCAGGCACTGGAGCTGAACCAGCGTGCCGGCACGGCCTTGATGCCCAAAATCCATATT
CTGGATTTAAAAGTTGCGCAAAAAAAGCATGGGATTAGCCTGCAATTGATTCAGGACATCAAAAAGCGCCTGGAGAAAAA
AGAACAGGTACTGATTTTTTTAAACCGCCGTGGCTATGCCCCCGTCCTGCTTTGTGGCAGCTGTGGCTGGCAGGCGCAAT
GTCCGCACTGTGATGCCAACTTCACTGTACACCGTCAGCCTTATCAGCATCTGCATTGCCATCACTGCGGTACTATTCAC
CGCATGCCGGAACACTGCCCGCAATGTCAGCATCAGGAACTGGTTACACTGGGTATGGGCACCGGCAAAGTCGAAGAACA
TTTAAATGAGCTATTTCCGGATTTTGAGGTGATTCGGGTCGATCGCGATTCCACCAGTCGGGTCGGCAGCTGGCAAAAAA
TCTATGACAAGATTCAGAAAAGCGAACCGGCGATTCTACTCGGCACCCAGATGCTGGCCAAAGGCCATCATTTTCCTTAT
GTGACGCTGGTGGCGATTCTGGATATCGATTCCGGTCTGCTGAGTGTGGATTTCCGTGCTACTGAACGGACTGCACAACT
GATCGTGCAGGTTGCAGGCCGTGCCGGACGTGGTGAACACAAAGGTGATGTCTATCTGCAAACGCTCAGACCAGATCATG
CCCTACTGAATACGCTGGTCAATGAAAATTATCGCGTATTTGCCCAGCAAACCTTAAAAGAACGGCAGATGGCCAAAATG
CCGCCCTATCGTTATGCCATCCTGATTCGCTGTGAATCCAAGGATCAGTCACAAAATACCGAATTCTTACAGAAACATGC
AGCACTCCTCAGGCAATATCCTGATCTGGCGCTGGACATCTGGGGACCGATTCCTGCGCCAATGGAACGCAAGGCGGGGC
GCTATCAGTCGCATATGGTGCTATTGTCAGCGGATCGTCCACGTTTGCATTACTATGTGCGTAGCTGGTGGCAAAACATG
CTGCAGGACAAACCTTCTAGCATGAAGCTCACGCTGGACATCGACCCTCAGGAACTGAGCTAA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(15-114)

Type III restriction enzyme, res subunit (ResIII)

(209-369)

Primosomal protein N C-terminal domain (PriA_C)

(642-736)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

71.117

99.189

0.705