Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EVX74_RS13360 Genome accession   NZ_CP078045
Coordinates   2757396..2758196 (-) Length   266 a.a.
NCBI ID   WP_071850435.1    Uniprot ID   A0AAJ4P2Y2
Organism   Acinetobacter lwoffii strain AL_065     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2752396..2763196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVX74_RS13340 (EVX74_013250) - 2753441..2754139 (-) 699 WP_004278352.1 YfgM family protein -
  EVX74_RS13345 (EVX74_013255) hisS 2754170..2755465 (-) 1296 WP_129716397.1 histidine--tRNA ligase -
  EVX74_RS13350 (EVX74_013260) ispG 2755469..2756590 (-) 1122 WP_129716395.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EVX74_RS13355 (EVX74_013265) - 2756614..2757405 (-) 792 WP_129716394.1 helix-turn-helix domain-containing protein -
  EVX74_RS13360 (EVX74_013270) pilF 2757396..2758196 (-) 801 WP_071850435.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EVX74_RS13365 (EVX74_013275) rlmN 2758211..2759446 (-) 1236 WP_005107875.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EVX74_RS13370 (EVX74_013280) ndk 2759569..2760000 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  EVX74_RS13375 (EVX74_013285) iscX 2760111..2760311 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  EVX74_RS13380 (EVX74_013290) - 2760399..2760950 (-) 552 WP_005107877.1 phosphatase PAP2 family protein -
  EVX74_RS13385 (EVX74_013295) - 2760971..2762251 (-) 1281 WP_129716392.1 glycosyltransferase family 4 protein -
  EVX74_RS13390 (EVX74_013300) - 2762523..2762933 (-) 411 WP_129716390.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30133.26 Da        Isoelectric Point: 9.2878

>NTDB_id=586518 EVX74_RS13360 WP_071850435.1 2757396..2758196(-) (pilF) [Acinetobacter lwoffii strain AL_065]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWIGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=586518 EVX74_RS13360 WP_071850435.1 2757396..2758196(-) (pilF) [Acinetobacter lwoffii strain AL_065]
TTGAGAAATCTAACATCAAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGCGCACTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCCAATCGCGATTCCTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCAGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGGCAGAAAAATCAGG
GTGCCCGTGCACTCTGGATTGGCATTCGCACTGCGCGTGCCGGAGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

97.744

0.771
  pilF Acinetobacter baylyi ADP1

69.925

100

0.699