Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EGT68_RS12425 Genome accession   NZ_CP078042
Coordinates   2618983..2619783 (-) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ_068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2613983..2624783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT68_RS12405 (EGT68_012405) - 2615042..2615740 (-) 699 WP_004950316.1 tetratricopeptide repeat protein -
  EGT68_RS12410 (EGT68_012410) hisS 2615772..2617064 (-) 1293 WP_039047056.1 histidine--tRNA ligase -
  EGT68_RS12415 (EGT68_012415) ispG 2617061..2618176 (-) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EGT68_RS12420 (EGT68_012420) - 2618201..2618992 (-) 792 WP_042891256.1 RodZ domain-containing protein -
  EGT68_RS12425 (EGT68_012425) pilF 2618983..2619783 (-) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EGT68_RS12430 (EGT68_012430) rlmN 2619800..2621035 (-) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EGT68_RS12435 (EGT68_012435) ndk 2621157..2621588 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  EGT68_RS12440 (EGT68_012440) iscX 2621695..2621895 (-) 201 WP_042891252.1 Fe-S cluster assembly protein IscX -
  EGT68_RS12445 (EGT68_012445) - 2621978..2622529 (-) 552 WP_125272442.1 phosphatase PAP2 family protein -
  EGT68_RS12450 (EGT68_012450) - 2622557..2623873 (-) 1317 WP_171133351.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=586475 EGT68_RS12425 WP_004908991.1 2618983..2619783(-) (pilF) [Acinetobacter junii strain AJ_068]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=586475 EGT68_RS12425 WP_004908991.1 2618983..2619783(-) (pilF) [Acinetobacter junii strain AJ_068]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGCTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68