Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EGK58_RS12700 Genome accession   NZ_CP078027
Coordinates   2665504..2666304 (-) Length   266 a.a.
NCBI ID   WP_005232134.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain AV_175     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2660504..2671304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGK58_RS12680 (EGK58_012680) - 2661555..2662253 (-) 699 WP_005232125.1 YfgM family protein -
  EGK58_RS12685 (EGK58_012685) hisS 2662284..2663579 (-) 1296 WP_125279599.1 histidine--tRNA ligase -
  EGK58_RS12690 (EGK58_012690) ispG 2663583..2664704 (-) 1122 WP_004782104.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EGK58_RS12695 (EGK58_012695) - 2664728..2665513 (-) 786 WP_125279598.1 helix-turn-helix domain-containing protein -
  EGK58_RS12700 (EGK58_012700) pilF 2665504..2666304 (-) 801 WP_005232134.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EGK58_RS12705 (EGK58_012705) rlmN 2666319..2667554 (-) 1236 WP_005232136.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EGK58_RS12710 (EGK58_012710) ndk 2667676..2668107 (-) 432 WP_004782097.1 nucleoside-diphosphate kinase -
  EGK58_RS12715 (EGK58_012715) iscX 2668217..2668417 (-) 201 WP_005232138.1 Fe-S cluster assembly protein IscX -
  EGK58_RS12720 (EGK58_012720) - 2668506..2669057 (-) 552 WP_004782094.1 phosphatase PAP2 family protein -
  EGK58_RS12725 (EGK58_012725) - 2670030..2670731 (-) 702 WP_001223318.1 IS1-like element ISPa14 family transposase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29990.11 Da        Isoelectric Point: 9.0592

>NTDB_id=586425 EGK58_RS12700 WP_005232134.1 2665504..2666304(-) (pilF) [Acinetobacter variabilis strain AV_175]
MSNLTSKLALVSVVCGSLVLTACQTPDLGQKDPEKATKVRTQLAVEYLKNGDLDSAKRALDQALETNPRDSQANMMMGVL
LQQEGSKPNLEKAEGYFKRAISADPKNAQARNNYGTYLYQLERYKDAIEQFEIAGATLGYDQRFRALENMGRIYLKQGDL
VNAEKSFKQALQANRASYISMLELAEIFYLNQQFPAATQMYEQFVRGVGQKNQSARALWIGIRTARASGDQLGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=586425 EGK58_RS12700 WP_005232134.1 2665504..2666304(-) (pilF) [Acinetobacter variabilis strain AV_175]
TTGAGTAATCTGACATCTAAACTTGCTTTGGTTTCAGTAGTATGTGGGTCTTTGGTTTTAACTGCGTGTCAAACGCCAGA
TCTGGGTCAGAAGGACCCGGAAAAAGCCACCAAGGTTCGTACTCAGTTGGCGGTTGAATATCTTAAAAACGGTGATCTGG
ATTCAGCTAAACGTGCGCTGGATCAGGCACTGGAAACTAATCCGCGTGATTCGCAAGCCAATATGATGATGGGCGTGCTG
CTTCAGCAGGAAGGCAGCAAACCTAATCTTGAAAAGGCCGAAGGTTATTTCAAGCGTGCAATTTCGGCAGATCCAAAAAA
TGCCCAGGCACGAAATAACTACGGCACCTATTTATATCAGCTTGAGCGCTATAAAGATGCGATCGAGCAGTTCGAAATTG
CCGGCGCAACTTTAGGTTATGACCAGCGATTTCGTGCCCTTGAAAATATGGGACGGATTTACCTGAAACAGGGTGATTTG
GTGAATGCTGAAAAATCATTCAAACAGGCACTACAGGCTAATCGCGCTTCGTATATATCAATGTTAGAGTTGGCAGAAAT
TTTTTATCTGAACCAGCAGTTCCCGGCCGCGACTCAAATGTATGAACAGTTTGTACGTGGCGTGGGGCAGAAAAACCAGA
GTGCCCGTGCGCTCTGGATTGGCATTCGCACTGCGCGTGCCAGTGGCGATCAGTTAGGCATGCAGGTATTGGTCAATCAA
TTACGTGCATTGTTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(145-174)

Tetratricopeptide repeat (TPR_14)

(40-79)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.692

100

0.767
  pilF Acinetobacter baylyi ADP1

69.663

100

0.699