Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EGT69_RS03050 Genome accession   NZ_CP078018
Coordinates   644953..645753 (+) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ_351     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 639953..650753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT69_RS03025 (EGT69_003025) - 640863..642179 (+) 1317 WP_171133351.1 glycosyltransferase family 4 protein -
  EGT69_RS03030 (EGT69_003030) - 642207..642758 (+) 552 WP_087539381.1 phosphatase PAP2 family protein -
  EGT69_RS03035 (EGT69_003035) iscX 642841..643041 (+) 201 WP_042891252.1 Fe-S cluster assembly protein IscX -
  EGT69_RS03040 (EGT69_003040) ndk 643148..643579 (+) 432 WP_125297722.1 nucleoside-diphosphate kinase -
  EGT69_RS03045 (EGT69_003045) rlmN 643701..644936 (+) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EGT69_RS03050 (EGT69_003050) pilF 644953..645753 (+) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EGT69_RS03055 (EGT69_003055) - 645744..646535 (+) 792 WP_042891256.1 helix-turn-helix domain-containing protein -
  EGT69_RS03060 (EGT69_003060) ispG 646560..647675 (+) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EGT69_RS03065 (EGT69_003065) hisS 647672..648964 (+) 1293 WP_039047056.1 histidine--tRNA ligase -
  EGT69_RS03070 (EGT69_003070) - 648996..649694 (+) 699 WP_004950316.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=586370 EGT69_RS03050 WP_004908991.1 644953..645753(+) (pilF) [Acinetobacter junii strain AJ_351]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=586370 EGT69_RS03050 WP_004908991.1 644953..645753(+) (pilF) [Acinetobacter junii strain AJ_351]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGCTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68