Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KU515_RS05255 Genome accession   NZ_CP077932
Coordinates   1065722..1066696 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain 194     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1060722..1071696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU515_RS05215 (KU515_05200) gcvT 1061223..1062314 (-) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  KU515_RS05220 (KU515_05205) - 1062473..1062997 (-) 525 WP_001015120.1 shikimate kinase -
  KU515_RS05225 (KU515_05210) - 1062987..1063133 (-) 147 WP_001789879.1 hypothetical protein -
  KU515_RS05230 (KU515_05215) comGF 1063230..1063727 (-) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU515_RS05235 (KU515_05220) comGE 1063645..1063944 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU515_RS05240 (KU515_05225) comGD 1063931..1064377 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU515_RS05245 (KU515_05230) comGC 1064355..1064666 (-) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  KU515_RS05250 (KU515_05235) comGB 1064680..1065750 (-) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU515_RS05255 (KU515_05240) comGA 1065722..1066696 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU515_RS05260 (KU515_05245) - 1066748..1067371 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  KU515_RS05265 (KU515_05250) - 1067368..1067697 (-) 330 WP_001018870.1 MTH1187 family thiamine-binding protein -
  KU515_RS05270 (KU515_05255) - 1067697..1068683 (-) 987 WP_000161307.1 ROK family glucokinase -
  KU515_RS05275 (KU515_05260) - 1068680..1068883 (-) 204 WP_000087561.1 YqgQ family protein -
  KU515_RS05280 (KU515_05265) - 1068864..1070327 (-) 1464 WP_001017870.1 rhomboid family protein -
  KU515_RS05285 (KU515_05270) - 1070339..1070878 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  KU515_RS05290 (KU515_05275) rpmG 1071087..1071236 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=585500 KU515_RS05255 WP_000697220.1 1065722..1066696(-) (comGA) [Staphylococcus aureus strain 194]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=585500 KU515_RS05255 WP_000697220.1 1065722..1066696(-) (comGA) [Staphylococcus aureus strain 194]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTGATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1