Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KU536_RS05830 Genome accession   NZ_CP077924
Coordinates   1104865..1105839 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain 200     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1099865..1110839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU536_RS05795 (KU536_05765) rpmG 1100325..1100474 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  KU536_RS05800 (KU536_05770) - 1100683..1101222 (+) 540 WP_000164554.1 5-formyltetrahydrofolate cyclo-ligase -
  KU536_RS05805 (KU536_05775) - 1101234..1102697 (+) 1464 WP_001017856.1 rhomboid family protein -
  KU536_RS05810 (KU536_05780) - 1102678..1102881 (+) 204 WP_000087559.1 YqgQ family protein -
  KU536_RS05815 (KU536_05785) - 1102878..1103864 (+) 987 WP_000161315.1 ROK family glucokinase -
  KU536_RS05820 (KU536_05790) - 1103864..1104193 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KU536_RS05825 (KU536_05795) - 1104190..1104813 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KU536_RS05830 (KU536_05800) comGA 1104865..1105839 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU536_RS05835 (KU536_05805) comGB 1105811..1106881 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU536_RS05840 (KU536_05810) comGC 1106895..1107205 (+) 311 Protein_1114 competence type IV pilus major pilin ComGC -
  KU536_RS05845 (KU536_05815) comGD 1107183..1107629 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU536_RS05850 (KU536_05820) comGE 1107616..1107915 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU536_RS05855 (KU536_05825) comGF 1107833..1108330 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU536_RS05860 (KU536_05830) - 1108427..1108573 (+) 147 WP_001789879.1 hypothetical protein -
  KU536_RS05865 (KU536_05835) - 1108563..1109087 (+) 525 WP_001015120.1 shikimate kinase -
  KU536_RS05870 (KU536_05840) gcvT 1109246..1110337 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=585291 KU536_RS05830 WP_000697220.1 1104865..1105839(+) (comGA) [Staphylococcus aureus strain 200]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=585291 KU536_RS05830 WP_000697220.1 1104865..1105839(+) (comGA) [Staphylococcus aureus strain 200]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTACGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1